Yüksek Lisans Tezleri
Permanent URI for this collectionhttps://hdl.handle.net/20.500.12573/5799
Browse
3 results
Search Results
Master Thesis Tasarlanmış Mikroorganizmalar ile Katma Değeri Yüksek Karotenoidlerin Biyosentezi(Abdullah Gül Üniversitesi, Fen Bilimleri Enstitüsü, 2024) Arslansoy, Nuriye; Fidan, ÖzkanCarotenoids are pigment molecules that play an important role in coloring plants, algae, and other organisms. These molecules exhibit various biological activities such as anticancer, antiviral and antioxidant activities. They have a huge market size and are mainly used in the food, feed, and cosmetic industries. The current supply chain for carotenoids is mostly relied on the extraction from plants and/or chemical synthesis for certain carotenoids. However, these strategies have various bottlenecks and disadvantages such as being affected by climate change, more difficult and costly extraction processes, and environmental issues. These can be overcome with microbial biosynthesis, which not only addresses the previous problems but also provides advantages of producing in a short time and scale-up for industrial production. In this research, we aimed to biosynthesize the high value-added carotenoids by engineered microorganisms. The genome of a native producer of zeaxanthin diglucoside, identified as endophytic Pseudomonas sp. 102515, was first edited by CRISPR-Cas9 to knock out zeaxanthin glucosyltransferase (CrtX), lycopene β-cyclase (CrtY) and beta-carotene hydroxylase (CrtZ). This led to ΔcrtX, ΔcrtY and ΔcrtZ mutant strains of Pseudomonas sp. 102515. On the other hand, overexpression plasmids carrying crtW, CaZEP and CaZEP-CaCCSm40 genes were constructed and transformed to ΔcrtX mutant to synthesize astaxanthin, violaxanthin and capsanthin/capsorubin. HPLC analysis of extracts from mutant strains and overexpression strains revealed that all the engineered strains produced the corresponding carotenoids such as zeaxanthin, β-carotene, and lycopene. Thus, this study paved the way for the biosynthesis of valuable carotenoids in the engineered endophytic bacteria.Master Thesis BBSome, Caenorhabditis Elegans'ta ARL13B'ye Bağımlı İki Farklı Silyanın Birlikte Uzamasını Düzenler(Abdullah Gül Üniversitesi / Fen Bilimleri Enstitüsü, 2023) Turan, Merve Gül; Kaplan, Sebiha ÇevikCilia or flagella are interchangeably used to refer to the hair-like organelles extending from the cell surface to communicate with environmental signals or triggers. Cilium, the singular form of cilia, and its components are well-conserved structures throughout evolution and are divided into motile and primary cilium. The primary cilia of different cells are seen to form joint cilia by extending in parallel. For instance, PHA and PHB primary cilia in C. elegans protrude from the ends of the dendrite but extend parallel to one another and intersect in the middle portion of the cilia, reaching the same length. Nevertheless, the molecular mechanisms underlying how parallel cilia get similar lengths remain mysterious. In this thesis, we used C. elagans as a model organism to examine the molecular mechanism associated with the cilia direction. We generated various single, double, and triple mutants to examine PHA and PHB cilia for phenotype and length. We found that a Joubert syndrome protein, ARL13B, is required for determining cilia direction in PHA & PHB cilia and ASE & ASI cilia.Master Thesis Yeni Bir Silya Geni Olan TMEM145'in Karakterizasyonu(Abdullah Gül Üniversitesi, Fen Bilimleri Enstitüsü, 2020) Pir, Mustafa Samet; Kaplan, Oktay İsmailSilya ve flagella çoğu organizmada bulunan, mikrotübül yapılı, yüksek korunumlu hücresel bir yapıdır. Bunlar, protozoalarda hareket sağlamadan, çok hücreli canlılarda sinyal iletimine kadar bir çok fonksiyona sahiptir. Silyanın yapısında veya fonksiyonunda meydana gelen bozulmalar insanlarda silyopati denilen çeşitli hastalıklara sebep olur. Silyada meydana gelen bu bozukluklar silya genlerinde veya silya fonksiyonunu etkileyen silya geni olmayan genlerde meydana gelen mutasyonlardan kaynaklanır. Bu yüzden silyopatilerin moleküler temelini ortaya çıkarmaya yardımcı olacak yeni silya genleri keşfetmeye ihtiyaç vardır. GPCR proteini olan, insan TMEM145 geninin ortoloğu olan ve Caenorhabditis elegans'ta bulunan C15A7.2 genini silya geni olarak tanımladık. C15A7.2 geni tarafından kodlanan TMEM-145 proteinin fonksiyonunu araştırdık ve C15A7.2 mutantlarda intraflagellar transport sisteminin hızının yavaşladığını bulduk. Ne tekli, ne de çeşitli çiftli mutantlarda herhangi bir yapısal bozukluk gözlemlemedik. Bu da TMEM-145'in silya yapımında görev almadığını gösteriyor. Silyada bulunan bu genin tam fonksiyonunu öğrenmek için ilave analizler yapılmalıdır.