Protocol for cell surface biotinylation of magnetic labeled and captured human peripheral blood mononuclear cells
| dc.contributor.author | Ayaz-Guner, Serife | |
| dc.contributor.author | Acar, Mustafa Burak | |
| dc.contributor.author | Boyvat, Dudu | |
| dc.contributor.author | Guner, Huseyin | |
| dc.contributor.author | Bozalan, Habibe | |
| dc.contributor.author | Güzel, Melis | |
| dc.contributor.author | Yıldır, Selin Kübra | |
| dc.contributor.author | Altınsoy, Nilay | |
| dc.contributor.author | Fındık, Fatma | |
| dc.contributor.author | Karakükçü, Musa | |
| dc.contributor.author | Özcan, Servet | |
| dc.contributor.authorID | 0000-0002-1052-0961 | en_US |
| dc.contributor.authorID | 0000-0002-0220-5224 | en_US |
| dc.contributor.department | AGÜ, Yaşam ve Doğa Bilimleri Fakültesi, Moleküler Biyoloji ve Genetik Bölümü | en_US |
| dc.contributor.institutionauthor | Boyvat, Dudu | |
| dc.contributor.institutionauthor | Ayaz-Guner, Şerife | |
| dc.contributor.institutionauthor | Guner, Huseyin | |
| dc.date.accessioned | 2024-04-29T07:01:25Z | |
| dc.date.available | 2024-04-29T07:01:25Z | |
| dc.date.issued | 2022 | en_US |
| dc.description.abstract | Analysis of the surfaceome of a blood cell subset requires cell sorting, followed by surface protein enrichment. Here, we present a protocol combining magnetically activated cell sorting (MACS) and surface biotinylation of the target cell subset from human peripheral blood mononuclear cells (PBMCs). We describe the steps for isolating target cells and their in-column surface biotinylation, followed by isolation and mass spectrometry analysis of biotinylated proteins. The protocol enables in-column surface biotinylation of specific cell subsets with minimal membrane disruption. | en_US |
| dc.description.sponsorship | This work wassupportedbyTheScientificandTechnologicalResearchCouncilofTu¨rkiye(TU ¨BITAK) (Project Number: 119S457) to S.O. We thank Prof. Dr. Eugene STEELE for language editing. The graphical abstract and figures were created with BioRender.com. | en_US |
| dc.identifier.endpage | 15 | en_US |
| dc.identifier.issn | 2666-1667 | |
| dc.identifier.issue | 4 | en_US |
| dc.identifier.startpage | 1 | en_US |
| dc.identifier.uri | https://doi.org/10.1016/j.xpro.2022.101863 | |
| dc.identifier.uri | https://hdl.handle.net/20.500.12573/2118 | |
| dc.identifier.volume | 3 | en_US |
| dc.language.iso | eng | en_US |
| dc.publisher | Cell Press | en_US |
| dc.relation.isversionof | 10.1016/j.xpro.2022.101863 | en_US |
| dc.relation.journal | STAR Protocols | en_US |
| dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
| dc.relation.tubitak | 119S457 | |
| dc.rights | info:eu-repo/semantics/openAccess | en_US |
| dc.subject | Cell Biology | en_US |
| dc.subject | Cell Isolation | en_US |
| dc.subject | Mass Spectrometry | en_US |
| dc.subject | Protein Biochemistry | en_US |
| dc.subject | Proteomics | en_US |
| dc.title | Protocol for cell surface biotinylation of magnetic labeled and captured human peripheral blood mononuclear cells | en_US |
| dc.type | article | en_US |
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