WDR31 Displays Functional Redundancy With GTpase-Activating Proteins (GAPs) ELMOD and RP2 in Regulating Ift Complex and Recruiting the BBsome to Cilium
| dc.contributor.author | Cevik, Sebiha | |
| dc.contributor.author | Peng, Xiaoyu | |
| dc.contributor.author | Beyer, Tina | |
| dc.contributor.author | Pir, Mustafa S. | |
| dc.contributor.author | Yenisert, Ferhan | |
| dc.contributor.author | Woerz, Franziska | |
| dc.contributor.author | Kaplan, Oktay, I | |
| dc.date.accessioned | 2025-09-25T11:00:56Z | |
| dc.date.available | 2025-09-25T11:00:56Z | |
| dc.date.issued | 2023 | |
| dc.description | Beyer, Tina/0000-0002-6302-8997; Cakiroglu, Miray/0000-0002-4289-8344; Oner, Sukru Sadik/0000-0002-8864-7356; Woerz, Franziska/0009-0007-5484-358X; Kaplan, Oktay Ismail/0000-0002-8733-0920; Hoffmann, Felix/0000-0001-5199-2749; Yenisert, Ferhan/0000-0002-1028-8197; Pir, Mustafa Samet/0000-0002-4645-7626; Cevik, Sebiha/0000-0002-0935-1929 | en_US |
| dc.description.abstract | The correct intraflagellar transport (IFT) assembly at the ciliary base and the IFT turnaround at the ciliary tip are key for the IFT to perform its function, but we still have poor understanding about how these processes are regulated. Here, we identify WDR31 as a new ciliary protein, and analysis from zebrafish and Caeno-rhabditis elegans reveals the role of WDR31 in regulating the cilia morphology. We find that loss of WDR-31 together with RP-2 and ELMD-1 (the sole ortholog ELMOD1-3) results in ciliary accumu-lations of IFT Complex B components and KIF17 kinesin, with fewer IFT/BBSome particles traveling along cilia in both anterograde and retrograde directions, suggesting that the IFT/BBSome entry into the cilia and exit from the cilia are impacted. Furthermore, anterograde IFT in the middle segment travels at increased speed in wdr-31;rpi-2;elmd-1. Remarkably, a non-ciliary protein leaks into the cilia of wdr-31;rpi-2;elmd-1, possibly because of IFT de-fects. This work reveals WDR31-RP-2-ELMD-1 as IFT and BBSome trafficking regulators. | en_US |
| dc.description.sponsorship | National BioResource Project (NBRP) in Japan; NIH Of fice of Research Infrastructure Programs [P40 OD010440]; Abdullah Gul University Scienti fic Research Project Coordination Unit [TOA-2018-110]; Tistou AMP; Charlotte Kerstan Stiftung; Deutsche Forschungsgemeinschaft; [INST 2388/62-1] | en_US |
| dc.description.sponsorship | We thank Oliver Blacque, Piali Sengupta, Micheal Leroux, the National BioResource Project (NBRP) in Japan, and the CGC in the United States, which is financed by the NIH Of fice of Research Infrastructure Programs (P40 OD010440) for sharing valuable strains. We thank Atiyye Zorluer for gen- erating strains. We thank Oliver Blacque and Samuel Katz for critical reading of the manuscript. We thank the Abdullah Gul University Scienti fic Research Project Coordination Unit (Project number: TOA-2018-110) for providing the funding that initiated the project. M Uef fing was supported by the Tistou & Charlotte Kerstan Stiftung. The Leica laser scanning microscope was funded by a grant from Deutsche Forschungsgemeinschaft (INST 2388/62-1) . | en_US |
| dc.description.sponsorship | National BioResource Project (NBRP) in Japan; NIH Of fice of Research Infrastructure Programs [P40 OD010440]; Abdullah Gul University Scienti fic Research Project Coordination Unit [TOA-2018-110]; Tistou AMP; Charlotte Kerstan Stiftung; Deutsche Forschungsgemeinschaft; [INST 2388/62-1]; National Institute of General Medical Sciences; NIH Office of the Director [P40OD010440] Funding Source: NIH RePORTER | |
| dc.description.sponsorship | We thank Oliver Blacque, Piali Sengupta, Micheal Leroux, the National BioResource Project (NBRP) in Japan, and the CGC in the United States, which is financed by the NIH Office of Research Infrastructure Programs (P40 OD010440) for sharing valuable strains. We thank Atiyye Zorluer for generating strains. We thank Oliver Blacque and Samuel Katz for critical reading of the manuscript. We thank the Abdullah Gul University Scientific Research Project Coordination Unit (Project number: TOA-2018-110) for providing the funding that initiated the project. M Ueffing was supported by the Tistou & Charlotte Kerstan Stiftung. The Leica laser scanning microscope was funded by a grant from Deutsche Forschungsgemeinschaft (INST 2388/62-1). | |
| dc.description.sponsorship | Abdullah Gul University, (TOA-2018-110); National Institutes of Health, NIH, (P40 OD010440); Deutsche Forschungsgemeinschaft, DFG, (INST 2388/62-1); Tistou and Charlotte Kerstan Stiftung | |
| dc.identifier.doi | 10.26508/lsa.202201844 | |
| dc.identifier.issn | 2575-1077 | |
| dc.identifier.scopus | 2-s2.0-85159654316 | |
| dc.identifier.uri | https://doi.org/10.26508/lsa.202201844 | |
| dc.identifier.uri | https://hdl.handle.net/20.500.12573/4956 | |
| dc.language.iso | en | en_US |
| dc.publisher | Life Science Alliance Llc | en_US |
| dc.relation.ispartof | Life Science Alliance | en_US |
| dc.rights | info:eu-repo/semantics/openAccess | en_US |
| dc.title | WDR31 Displays Functional Redundancy With GTpase-Activating Proteins (GAPs) ELMOD and RP2 in Regulating Ift Complex and Recruiting the BBsome to Cilium | en_US |
| dc.type | Article | en_US |
| dspace.entity.type | Publication | |
| gdc.author.id | Beyer, Tina/0000-0002-6302-8997 | |
| gdc.author.id | Cakiroglu, Miray/0000-0002-4289-8344 | |
| gdc.author.id | Oner, Sukru Sadik/0000-0002-8864-7356 | |
| gdc.author.id | Woerz, Franziska/0009-0007-5484-358X | |
| gdc.author.id | Kaplan, Oktay Ismail/0000-0002-8733-0920 | |
| gdc.author.id | Hoffmann, Felix/0000-0001-5199-2749 | |
| gdc.author.id | Cevik, Sebiha/0000-0002-0935-1929 | |
| gdc.author.id | PİR, MUSTAFA SAMET/0000-0002-4645-7626 | |
| gdc.author.id | YENİSERT, FERHAN/0000-0002-1028-8197 | |
| gdc.author.scopusid | 23471639400 | |
| gdc.author.scopusid | 57226266920 | |
| gdc.author.scopusid | 15753596200 | |
| gdc.author.scopusid | 57222060293 | |
| gdc.author.scopusid | 57226277057 | |
| gdc.author.scopusid | 57226282736 | |
| gdc.author.scopusid | 57226291650 | |
| gdc.author.scopusid | 23472963100 | |
| gdc.author.wosid | Oner, Sukru/K-8032-2012 | |
| gdc.author.wosid | Peng, Xiaoyu/Iun-3010-2023 | |
| gdc.author.wosid | Beyer, Tina/Aag-1202-2021 | |
| gdc.author.wosid | Kaplan, Oktay Ismail/F-8531-2015 | |
| gdc.author.wosid | Pir, Mustafa/Aeo-1681-2022 | |
| gdc.author.wosid | Kaplan, Oktay/F-8531-2015 | |
| gdc.author.wosid | YENİSERT, FERHAN/PMR-2493-2026 | |
| gdc.bip.impulseclass | C4 | |
| gdc.bip.influenceclass | C5 | |
| gdc.bip.popularityclass | C4 | |
| gdc.coar.access | open access | |
| gdc.coar.type | text::journal::journal article | |
| gdc.collaboration.industrial | false | |
| gdc.description.department | Abdullah Gül University | en_US |
| gdc.description.departmenttemp | [Cevik, Sebiha; Pir, Mustafa S.; Yenisert, Ferhan; Altunkaynak, Betul; Pir, Betul; Kaplan, Oktay, I] Abdullah Gul Univ, Sch Life & Nat Sci, Rare Dis Lab, Kayseri, Turkiye; [Peng, Xiaoyu; Cao, Ying] Tongji Univ, Sch Life Sci & Technol, Shanghai, Peoples R China; [Beyer, Tina; Woerz, Franziska; Hoffmann, Felix] Univ Tubingen, Inst Ophthalm Res, Ctr Ophthalmol, Tubingen, Germany; [Oner, S. Sadik] Goztepe Prof Dr Suleyman Yalcin City Hosp, Istanbul, Turkiye; [Karaman, Asli; Cakiroglu, Miray; Oner, S. Sadik] Istanbul Medeniyet Univ, Sci & Adv Technol Applicat & Res Ctr, Istanbul, Turkiye | en_US |
| gdc.description.issue | 8 | en_US |
| gdc.description.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
| gdc.description.scopusquality | Q2 | |
| gdc.description.startpage | e202201844 | |
| gdc.description.volume | 6 | en_US |
| gdc.description.woscitationindex | Science Citation Index Expanded | |
| gdc.description.wosquality | Q2 | |
| gdc.identifier.openalex | W4377142795 | |
| gdc.identifier.pmid | 37208194 | |
| gdc.identifier.wos | WOS:000995885500002 | |
| gdc.index.type | WoS | |
| gdc.index.type | Scopus | |
| gdc.index.type | PubMed | |
| gdc.oaire.accesstype | GOLD | |
| gdc.oaire.diamondjournal | false | |
| gdc.oaire.downloads | 80 | |
| gdc.oaire.impulse | 7.0 | |
| gdc.oaire.influence | 2.6684737E-9 | |
| gdc.oaire.isgreen | true | |
| gdc.oaire.keywords | A COMPLEX | |
| gdc.oaire.keywords | RAFFICKING | |
| gdc.oaire.keywords | GTPase-Activating Proteins | |
| gdc.oaire.keywords | ZONE | |
| gdc.oaire.keywords | Biological Transport | |
| gdc.oaire.keywords | Zebrafish Proteins | |
| gdc.oaire.keywords | CILIARY GENES | |
| gdc.oaire.keywords | INTRAFLAGELLAR TRANSPORT | |
| gdc.oaire.keywords | PHOSPHOINOSITIDEST | |
| gdc.oaire.keywords | Animals | |
| gdc.oaire.keywords | Cilia | |
| gdc.oaire.keywords | CAENORHABDITIS-ELEGANS | |
| gdc.oaire.keywords | Caenorhabditis elegans | |
| gdc.oaire.keywords | Caenorhabditis elegans Proteins | |
| gdc.oaire.keywords | GENOMICS | |
| gdc.oaire.keywords | Research Articles | |
| gdc.oaire.keywords | Zebrafish | |
| gdc.oaire.keywords | CILIOGENESIS | |
| gdc.oaire.popularity | 7.2901813E-9 | |
| gdc.oaire.publicfunded | false | |
| gdc.oaire.views | 132 | |
| gdc.openalex.collaboration | International | |
| gdc.openalex.fwci | 2.5724 | |
| gdc.openalex.normalizedpercentile | 0.9 | |
| gdc.openalex.toppercent | TOP 10% | |
| gdc.opencitations.count | 6 | |
| gdc.plumx.mendeley | 5 | |
| gdc.plumx.pubmedcites | 3 | |
| gdc.plumx.scopuscites | 5 | |
| gdc.scopus.citedcount | 5 | |
| gdc.virtual.author | Çevik Kaplan, Sebiha | |
| gdc.virtual.author | Kaplan, Oktay İsmail | |
| gdc.wos.citedcount | 5 | |
| relation.isAuthorOfPublication | 54165f6a-de67-4e5d-aafa-f109790f8b4c | |
| relation.isAuthorOfPublication | b48fbd00-611e-4077-99b7-3fba0f03d2ae | |
| relation.isAuthorOfPublication.latestForDiscovery | 54165f6a-de67-4e5d-aafa-f109790f8b4c | |
| relation.isOrgUnitOfPublication | 665d3039-05f8-4a25-9a3c-b9550bffecef | |
| relation.isOrgUnitOfPublication | 4eea69bf-e8aa-4e3e-ab18-7587ac1d841b | |
| relation.isOrgUnitOfPublication | f1a6e7de-5c27-471c-ada8-77b7e5558b19 | |
| relation.isOrgUnitOfPublication | 5519c95e-5bcb-45e5-8ce1-a8b4bcf7c7b9 | |
| relation.isOrgUnitOfPublication.latestForDiscovery | 665d3039-05f8-4a25-9a3c-b9550bffecef |