Protocol for Cell Surface Biotinylation of Magnetic Labeled and Captured Human Peripheral Blood Mononuclear Cells
| dc.contributor.author | Ayaz-Guner, Serife | |
| dc.contributor.author | Acar, Mustafa Burak | |
| dc.contributor.author | Boyvat, Dudu | |
| dc.contributor.author | Guner, Huseyin | |
| dc.contributor.author | Bozalan, Habibe | |
| dc.contributor.author | Guzel, Melis | |
| dc.contributor.author | Ozcan, Servet | |
| dc.date.accessioned | 2025-09-25T10:55:46Z | |
| dc.date.available | 2025-09-25T10:55:46Z | |
| dc.date.issued | 2022 | |
| dc.description | Guner, Huseyin/0000-0002-0220-5224; Findik, Fatma/0000-0001-9165-3653; Ayaz Guner, Serife/0000-0002-1052-0961; Acar, Mustafa Burak/0000-0002-9109-6575; Guzel, Melis/0000-0002-5973-0839; | en_US |
| dc.description.abstract | Analysis of the surfaceome of a blood cell subset requires cell sorting, followed by surface protein enrichment. Here, we present a protocol combining magnet-ically activated cell sorting (MACS) and surface biotinylation of the target cell subset from human peripheral blood mononuclear cells (PBMCs). We describe the steps for isolating target cells and their in-column surface biotinylation, fol-lowed by isolation and mass spectrometry analysis of biotinylated proteins. The protocol enables in-column surface biotinylation of specific cell subsets with minimal membrane disruption. | en_US |
| dc.description.sponsorship | Scientific and Technological Research Council of Turkiye (TUBITAK) [119S457] | en_US |
| dc.description.sponsorship | This work was supported by The Scientific and Technological Research Council of Turkiye (TUBITAK) (Project Number: 119S457) to S.O. We thank Prof. Dr. Eugene STEELE for language editing. The graphical abstract and figures were created with BioRender.com . | en_US |
| dc.description.sponsorship | Türkiye Bilimsel ve Teknolojik Araştırma Kurumu, TÜBİTAK, (119S457) | |
| dc.identifier.doi | 10.1016/j.xpro.2022.101863 | |
| dc.identifier.issn | 2666-1667 | |
| dc.identifier.scopus | 2-s2.0-85142326332 | |
| dc.identifier.uri | https://doi.org/10.1016/j.xpro.2022.101863 | |
| dc.identifier.uri | https://hdl.handle.net/20.500.12573/4501 | |
| dc.language.iso | en | en_US |
| dc.publisher | Elsevier | en_US |
| dc.relation.ispartof | Star Protocols | en_US |
| dc.rights | info:eu-repo/semantics/openAccess | en_US |
| dc.subject | Mass Spectrometry | |
| dc.subject | Cell Biology | |
| dc.subject | Protein Biochemistry | |
| dc.subject | Proteomics | |
| dc.subject | Cell Isolation | |
| dc.title | Protocol for Cell Surface Biotinylation of Magnetic Labeled and Captured Human Peripheral Blood Mononuclear Cells | en_US |
| dc.type | Article | en_US |
| dspace.entity.type | Publication | |
| gdc.author.id | Guner, Huseyin/0000-0002-0220-5224 | |
| gdc.author.id | Findik, Fatma/0000-0001-9165-3653 | |
| gdc.author.id | Ayaz Guner, Serife/0000-0002-1052-0961 | |
| gdc.author.id | Acar, Mustafa Burak/0000-0002-9109-6575 | |
| gdc.author.id | Guzel, Melis/0000-0002-5973-0839 | |
| gdc.author.id | BOZALAN, Habibe Damla/0000-0001-6556-880X | |
| gdc.author.scopusid | 33567596300 | |
| gdc.author.scopusid | 57006292300 | |
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| gdc.author.scopusid | 57972589200 | |
| gdc.author.scopusid | 26644286400 | |
| gdc.author.wosid | Bozalan, Damla/Oen-6613-2025 | |
| gdc.author.wosid | Guner, Huseyin/E-3323-2018 | |
| gdc.author.wosid | Acar, M. Burak/Aam-6407-2021 | |
| gdc.author.wosid | Ozcan, Servet/Aaa-7801-2022 | |
| gdc.author.wosid | Ayaz-Guner, Serife/K-4139-2019 | |
| gdc.author.wosid | Karakukcu, Musa/Aat-4574-2021 | |
| gdc.bip.impulseclass | C5 | |
| gdc.bip.influenceclass | C5 | |
| gdc.bip.popularityclass | C4 | |
| gdc.coar.access | open access | |
| gdc.coar.type | text::journal::journal article | |
| gdc.collaboration.industrial | false | |
| gdc.description.department | Abdullah Gül University | en_US |
| gdc.description.departmenttemp | [Ayaz-Guner, Serife; Findik, Fatma] Izmir Inst Technol, Dept Mol Biol & Genet, Izmir, Turkey; [Ayaz-Guner, Serife; Guner, Huseyin] Abdullah Gul Univ, Fac Life & Nat Sci, Dept Mol Biol & Genet, Kayseri, Turkey; [Acar, Mustafa Burak; Ozcan, Servet] Erciyes Univ, Fac Sci, Dept Biol, TR-38039 Kayseri, Turkey; [Acar, Mustafa Burak; Bozalan, Habibe; Guzel, Melis; Yildir, Selin Kubra; Altinsoy, Nilay; Ozcan, Servet] Erciyes Univ, Genome & Stem Cell Ctr GenKok, TR-38280 Kayseri, Turkey; [Boyvat, Dudu] Abdullah Gul Univ, Grad Sch Engn & Sci, Bioengn Program, TR-38080 Kayseri, Turkey; [Bozalan, Habibe; Guzel, Melis; Altinsoy, Nilay] Erciyes Univ, Gevher Nesibe Genome & Stem Cell Inst, TR-38039 Kayseri, Turkey; [Yildir, Selin Kubra] Erciyes Univ, Inst Hlth Sci, TR-38039 Kayseri, Turkey; [Karakukcu, Musa] Erciyes Univ, KANKA Pediat Bone Marrow Transplantat Ctr, Fac Med, Dept Pediat Hematol & Oncol, TR-38039 Kayseri, Turkey | en_US |
| gdc.description.issue | 4 | en_US |
| gdc.description.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
| gdc.description.scopusquality | Q4 | |
| gdc.description.volume | 3 | en_US |
| gdc.description.woscitationindex | Emerging Sources Citation Index | |
| gdc.description.wosquality | Q4 | |
| gdc.identifier.openalex | W4309503254 | |
| gdc.identifier.pmid | 36595912 | |
| gdc.identifier.wos | WOS:001059416400008 | |
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| gdc.oaire.isgreen | true | |
| gdc.oaire.keywords | Proteomics | |
| gdc.oaire.keywords | Science (General) | |
| gdc.oaire.keywords | Magnetic Phenomena | |
| gdc.oaire.keywords | Cell Membrane | |
| gdc.oaire.keywords | Membrane Proteins | |
| gdc.oaire.keywords | Cell Biology | |
| gdc.oaire.keywords | Mass Spectrometry | |
| gdc.oaire.keywords | Q1-390 | |
| gdc.oaire.keywords | Protein Biochemistry | |
| gdc.oaire.keywords | Protocol | |
| gdc.oaire.keywords | Leukocytes, Mononuclear | |
| gdc.oaire.keywords | Cell Isolation | |
| gdc.oaire.keywords | Humans | |
| gdc.oaire.keywords | Biotinylation | |
| gdc.oaire.popularity | 4.7717106E-9 | |
| gdc.oaire.publicfunded | false | |
| gdc.oaire.sciencefields | 0301 basic medicine | |
| gdc.oaire.sciencefields | 0303 health sciences | |
| gdc.oaire.sciencefields | 03 medical and health sciences | |
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| gdc.plumx.pubmedcites | 1 | |
| gdc.plumx.scopuscites | 4 | |
| gdc.scopus.citedcount | 4 | |
| gdc.virtual.author | Güner, Hüseyin | |
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