Protocol for Determining the Average Speed and Frequency of Kinesin and Dynein-Driven Intraflagellar Transport (IFT) in C. Elegans
| dc.contributor.author | Turan, Merve G. | |
| dc.contributor.author | Kantarci, Hanife | |
| dc.contributor.author | Temtek, Sadiye D. | |
| dc.contributor.author | Cakici, Onur | |
| dc.contributor.author | Cevik, Sebiha | |
| dc.contributor.author | Kaplan, Oktay, I | |
| dc.date.accessioned | 2025-09-25T10:55:47Z | |
| dc.date.available | 2025-09-25T10:55:47Z | |
| dc.date.issued | 2022 | |
| dc.description | Cakici, Onur/0000-0002-6809-4467; Kaplan, Oktay Ismail/0000-0002-8733-0920; Turan, Merve Gul/0000-0001-5783-7168 | en_US |
| dc.description.abstract | Here, we present a protocol to image a fluorescent-labeled intraflagellar trans-port (IFT) component in Caenorhabditis elegans with fluorescence microscopy, including steps of sample preparations, in vivo live-cell imaging, and post -micro-scopy analysis with kymographs. This protocol breaks down all processes into three categories: (1) pre-imaging preparations, (2) preparations for the time of image acquisition, and (3) post-imaging analyses. The protocol can be applied to determine the speed and frequency of moving particles. For complete details on the use and execution of this protocol, please refer to Cevik et al. (2021). | en_US |
| dc.description.sponsorship | TUBITAK [118S552] | en_US |
| dc.description.sponsorship | The Kaplan Lab is currently funded by TUBITAK (Project number: 118S552) . The graphical abstract was created with BioRender.com . | en_US |
| dc.description.sponsorship | Türkiye Bilimsel ve Teknolojik Araştırma Kurumu, TÜBİTAK, (118S552); Türkiye Bilimsel ve Teknolojik Araştırma Kurumu, TÜBİTAK | |
| dc.identifier.doi | 10.1016/j.xpro.2022.101498 | |
| dc.identifier.issn | 2666-1667 | |
| dc.identifier.scopus | 2-s2.0-85132922396 | |
| dc.identifier.uri | https://doi.org/10.1016/j.xpro.2022.101498 | |
| dc.identifier.uri | https://hdl.handle.net/20.500.12573/4502 | |
| dc.language.iso | en | en_US |
| dc.publisher | Elsevier | en_US |
| dc.relation.ispartof | Star Protocols | en_US |
| dc.rights | info:eu-repo/semantics/openAccess | en_US |
| dc.subject | Molecular Biology | |
| dc.subject | Cell Biology | |
| dc.subject | Genetics | |
| dc.subject | Model Organisms | |
| dc.subject | Neuroscience | |
| dc.subject | Bioinformatics | |
| dc.subject | Microscopy | |
| dc.subject | CRISPR | |
| dc.title | Protocol for Determining the Average Speed and Frequency of Kinesin and Dynein-Driven Intraflagellar Transport (IFT) in C. Elegans | en_US |
| dc.type | Article | en_US |
| dspace.entity.type | Publication | |
| gdc.author.id | Cakici, Onur/0000-0002-6809-4467 | |
| gdc.author.id | Kaplan, Oktay Ismail/0000-0002-8733-0920 | |
| gdc.author.id | Turan, Merve Gul/0000-0001-5783-7168 | |
| gdc.author.id | Kantarci, Hanife/0000-0003-4322-7101 | |
| gdc.author.scopusid | 59622840200 | |
| gdc.author.scopusid | 57765010300 | |
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| gdc.author.scopusid | 57765445200 | |
| gdc.author.scopusid | 23471639400 | |
| gdc.author.scopusid | 23472963100 | |
| gdc.author.wosid | Kaplan, Oktay Ismail/F-8531-2015 | |
| gdc.author.wosid | Kaplan, Oktay/F-8531-2015 | |
| gdc.author.wosid | Turan, Merve Gül/Jce-2657-2023 | |
| gdc.bip.impulseclass | C5 | |
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| gdc.coar.access | open access | |
| gdc.coar.type | text::journal::journal article | |
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| gdc.description.department | Abdullah Gül University | en_US |
| gdc.description.departmenttemp | [Turan, Merve G.; Kantarci, Hanife; Temtek, Sadiye D.; Cakici, Onur; Cevik, Sebiha; Kaplan, Oktay, I] Abdullah Gul Univ, Sch Life & Nat Sci, Rare Dis Lab, Kayseri, Turkey | en_US |
| gdc.description.issue | 3 | en_US |
| gdc.description.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
| gdc.description.scopusquality | Q4 | |
| gdc.description.volume | 3 | en_US |
| gdc.description.woscitationindex | Emerging Sources Citation Index | |
| gdc.description.wosquality | Q4 | |
| gdc.identifier.openalex | W4283371853 | |
| gdc.identifier.pmid | 35776634 | |
| gdc.identifier.wos | WOS:001059406300008 | |
| gdc.index.type | WoS | |
| gdc.index.type | Scopus | |
| gdc.index.type | PubMed | |
| gdc.oaire.accesstype | GOLD | |
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| gdc.oaire.keywords | Microscopy | |
| gdc.oaire.keywords | Science (General) | |
| gdc.oaire.keywords | Bioinformatics | |
| gdc.oaire.keywords | Dyneins | |
| gdc.oaire.keywords | Kinesins | |
| gdc.oaire.keywords | Cell Biology | |
| gdc.oaire.keywords | Q1-390 | |
| gdc.oaire.keywords | #cilia #IFT #celegans | |
| gdc.oaire.keywords | Model Organisms | |
| gdc.oaire.keywords | Flagella | |
| gdc.oaire.keywords | CRISPR | |
| gdc.oaire.keywords | Mutation | |
| gdc.oaire.keywords | Genetics | |
| gdc.oaire.keywords | Protocol | |
| gdc.oaire.keywords | Animals | |
| gdc.oaire.keywords | Cilia | |
| gdc.oaire.keywords | Caenorhabditis elegans | |
| gdc.oaire.keywords | Caenorhabditis elegans Proteins | |
| gdc.oaire.keywords | Molecular Biology | |
| gdc.oaire.keywords | Neuroscience | |
| gdc.oaire.popularity | 4.013858E-9 | |
| gdc.oaire.publicfunded | false | |
| gdc.oaire.sciencefields | 0301 basic medicine | |
| gdc.oaire.sciencefields | 0303 health sciences | |
| gdc.oaire.sciencefields | 03 medical and health sciences | |
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| gdc.scopus.citedcount | 2 | |
| gdc.virtual.author | Çevik Kaplan, Sebiha | |
| gdc.virtual.author | Kaplan, Oktay İsmail | |
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