Measurement of Autophagic Activity in Cancer Cells With Flow Cytometric Analysis Using Cyto-Id Staining
| dc.contributor.author | Şansaçar, Merve | |
| dc.contributor.author | Gencer Akçok, Emel Başak | |
| dc.date.accessioned | 2025-09-25T10:50:36Z | |
| dc.date.available | 2025-09-25T10:50:36Z | |
| dc.date.issued | 2025 | |
| dc.description.abstract | Autophagy is an evolutionarily conserved process providing the energy that cells need to survive, especially in stress situations, through catabolic processes. Considering the dual role of autophagy in cancer cells depending on the cellular context, it is crucial to comprehend the effect of drug candidates put forward to prevent cancer through the autophagy pathway. The CYTO-ID® Autophagy Detection Kit allows a rapid, specific and quantitative measurement of autophagic activity at the cellular level using a 488 nm-excitable green fluorescent detection reagent via flow cytometer. In this chapter, we present the CYTO-ID® Autophagy Detection method with a stepwise protocol to monitor the autophagy flux after the application of any compound to suspension cancer cell lines with flow cytometric analysis. © 2025 Elsevier B.V., All rights reserved. | en_US |
| dc.identifier.doi | 10.1007/7651_2024_526 | |
| dc.identifier.isbn | 9781597452946 | |
| dc.identifier.isbn | 9781617792304 | |
| dc.identifier.isbn | 9781617797668 | |
| dc.identifier.isbn | 1597455741 | |
| dc.identifier.isbn | 9781603272476 | |
| dc.identifier.isbn | 9781597453035 | |
| dc.identifier.isbn | 9781493912230 | |
| dc.identifier.isbn | 9781588298645 | |
| dc.identifier.isbn | 9781617793394 | |
| dc.identifier.isbn | 9781617799648 | |
| dc.identifier.issn | 1064-3745 | |
| dc.identifier.issn | 1940-6029 | |
| dc.identifier.scopus | 2-s2.0-85219494510 | |
| dc.identifier.uri | https://doi.org/10.1007/7651_2024_526 | |
| dc.identifier.uri | https://hdl.handle.net/20.500.12573/4175 | |
| dc.language.iso | en | en_US |
| dc.publisher | Humana Press Inc. | en_US |
| dc.relation.ispartof | Methods in Molecular Biology | en_US |
| dc.rights | info:eu-repo/semantics/closedAccess | en_US |
| dc.subject | Autophagic Compartments | en_US |
| dc.subject | Autophagic Flux | en_US |
| dc.subject | Autophagosome | en_US |
| dc.subject | Autophagy | en_US |
| dc.subject | Cancer | en_US |
| dc.subject | Cyto-Id | en_US |
| dc.subject | Flow Cytometry | en_US |
| dc.subject | Chloroquine | en_US |
| dc.subject | Sirolimus | en_US |
| dc.subject | Fluorescent Dyes | en_US |
| dc.subject | Cyto-Id | en_US |
| dc.subject | Chloroquine | en_US |
| dc.subject | Reagent | en_US |
| dc.subject | Sirolimus | en_US |
| dc.subject | Fluorescent Dye | en_US |
| dc.subject | Autolysosome | en_US |
| dc.subject | Autophagosome | en_US |
| dc.subject | Autophagy (Cellular) | en_US |
| dc.subject | Cancer Cell | en_US |
| dc.subject | Cell Culture | en_US |
| dc.subject | Flow Cytometry | en_US |
| dc.subject | Fluorescence Activated Cell Sorting | en_US |
| dc.subject | Fluorescence Intensity | en_US |
| dc.subject | Human | en_US |
| dc.subject | Human Cell | en_US |
| dc.subject | Chemistry | en_US |
| dc.subject | Metabolism | en_US |
| dc.subject | Neoplasm | en_US |
| dc.subject | Pathology | en_US |
| dc.subject | Procedures | en_US |
| dc.subject | Staining | en_US |
| dc.subject | Tumor Cell Line | en_US |
| dc.subject | Autophagy | en_US |
| dc.subject | Cell Line, Tumor | en_US |
| dc.subject | Flow Cytometry | en_US |
| dc.subject | Fluorescent Dyes | en_US |
| dc.subject | Humans | en_US |
| dc.subject | Neoplasms | en_US |
| dc.subject | Staining and Labeling | en_US |
| dc.title | Measurement of Autophagic Activity in Cancer Cells With Flow Cytometric Analysis Using Cyto-Id Staining | en_US |
| dc.type | Book Part | en_US |
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| gdc.description.department | Abdullah Gül University | en_US |
| gdc.description.departmenttemp | [Şansaçar] Merve, Department of Bioengineering, Abdullah Gül Üniversitesi, Kayseri, Turkey, Faculty of Engineering, Alanya Alaaddin Keykubat University, Alanya, Turkey; [Gencer Akçok] Emel Başak, Faculty of Life and Natural Sciences, Abdullah Gül Üniversitesi, Kayseri, Turkey | en_US |
| gdc.description.endpage | 224 | en_US |
| gdc.description.publicationcategory | Kitap Bölümü - Uluslararası | en_US |
| gdc.description.scopusquality | Q4 | |
| gdc.description.startpage | 219 | en_US |
| gdc.description.volume | 2879 | en_US |
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| gdc.oaire.keywords | Staining and Labeling | |
| gdc.oaire.keywords | Cell Line, Tumor | |
| gdc.oaire.keywords | Neoplasms | |
| gdc.oaire.keywords | Autophagy | |
| gdc.oaire.keywords | Humans | |
| gdc.oaire.keywords | Flow Cytometry | |
| gdc.oaire.keywords | Fluorescent Dyes | |
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| gdc.virtual.author | Şansaçar, Merve | |
| gdc.virtual.author | Gencer Akçok, Emel Başak | |
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