Green Magnetic Nanoparticles in Enzyme Immobilization

Abstract

Enzymes as highly efficient biocatalysts are widely used in various biotechnological sectors including cosmetic, textile, food, and detergent. However, their practical applications can be hampered due to high expense and challenges in the extraction, separation, and purification steps. Additionally, enzymes have limited activity and stability at high temperature and different pH values. Aiming to overcome these challenges, various nanoparticles (NPs) have been used as supporting matrices and carriers for enzyme immobilization. Among the NPs, in recent years, green magnetic nanoparticles (GMNPs) have attracted great attention as ideal support material for enzyme immobilization owing to their exceptional properties, such as their easy preparation, low toxicity, biocompatibility, and lower diffusion limitation. These properties enable the immobilized enzymes on GMNPs to be used in many different applications. This chapter aims to summarize the recent developments about enzyme immobilization on magnetic nanoparticles and GMNPs and their practical applications. © 2024 Elsevier B.V., All rights reserved.