Cebeci Aydın, Aysun
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Aydin, Aysun Cebeci
Aydın, Aysun Cebeci
Aysun Cebeci Aydın
CEBECİ Aysun
Cebeci, A.
Cebeci, Aysun
Aydın, Aysun Cebeci
Aysun Cebeci Aydın
CEBECİ Aysun
Cebeci, A.
Cebeci, Aysun
Job Title
Dr. Öğr. Üyesi
Email Address
aysun.cebeciaydin@agu.edu.tr
Main Affiliation
02.07. Malzeme Bilimi ve Nanoteknoloji Mühendisliği
Status
Current Staff
Website
ORCID ID
Scopus Author ID
Turkish CoHE Profile ID
Google Scholar ID
WoS Researcher ID
Sustainable Development Goals
13
CLIMATE ACTION
0
Research Products
17
PARTNERSHIPS FOR THE GOALS
0
Research Products
8
DECENT WORK AND ECONOMIC GROWTH
0
Research Products
9
INDUSTRY, INNOVATION AND INFRASTRUCTURE
0
Research Products
12
RESPONSIBLE CONSUMPTION AND PRODUCTION
0
Research Products
16
PEACE, JUSTICE AND STRONG INSTITUTIONS
0
Research Products
11
SUSTAINABLE CITIES AND COMMUNITIES
0
Research Products
1
NO POVERTY
0
Research Products
6
CLEAN WATER AND SANITATION
0
Research Products
10
REDUCED INEQUALITIES
0
Research Products
14
LIFE BELOW WATER
0
Research Products
15
LIFE ON LAND
0
Research Products
5
GENDER EQUALITY
0
Research Products
4
QUALITY EDUCATION
0
Research Products
7
AFFORDABLE AND CLEAN ENERGY
0
Research Products
3
GOOD HEALTH AND WELL-BEING
3
Research Products
2
ZERO HUNGER
0
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This researcher does not have a Scopus ID.
Documents
43
Citations
2409
Scholarly Output
11
Articles
10
Views / Downloads
460/288
Supervised MSc Theses
1
Supervised PhD Theses
0
WoS Citation Count
111
Scopus Citation Count
122
WoS h-index
4
Scopus h-index
5
Patents
0
Projects
1
WoS Citations per Publication
10.09
Scopus Citations per Publication
11.09
Open Access Source
7
Supervised Theses
1
| Journal | Count |
|---|---|
| Biocatalysis and Agricultural Biotechnology | 1 |
| Bioscience Biotechnology and Biochemistry | 1 |
| ÇOMÜ Ziraat Fakültesi Dergisi | 1 |
| Current Microbiology | 1 |
| Food Science and Biotechnology | 1 |
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11 results
Scholarly Output Search Results
Now showing 1 - 10 of 11
Article Citation - WoS: 48Citation - Scopus: 50Monitoring the Rhizopus Oryzae Lipase Catalyzed Hydrolysis of Castor Oil by ATR-FTIR Spectroscopy(Elsevier, 2015) Khaskheli, Abid Ali; Talpur, Farah N.; Ashraf, Muhammad Aqeel; Cebeci, Aysun; Jawaid, Sana; Afridi, Hassan ImranA rapid and environmental friendly Attenuated total reflectance Fourier-transform infrared (ATR-FTIR) spectroscopic method was developed for monitoring the Rhizopus oryzae lipase (ROL) catalyzed hydrolysis of castor oil in oil-in-water emulsion system. A calibration curve was constructed using partial least square (PLS) model by gravimetric addition of oleic acid (10-50%) in castor oil to detect the carbonyl absorption of free fatty acids (FFA) in the region (1690-1730 cm(-1)). The correlation co-efficient (R-2) and root mean square error of calibration (RMSEC) by PIS model were found to be 0.999 and 0.316, respectively. ROL was found to be an efficient biocatalyst to produce free fatty acids (FFA) from castor oil. Factors affecting the rate of hydrolysis such as enzyme concentration (0.01%, w/v), pH (7), temperature (37 degrees C), oil-water ratio (1:4) and reaction time (12 h) were optimized. Under all set of conditions the ROL effectively hydrolyzed castor oil up to 90% yield of fatty acids. The methodology is fairly environmental friendly in both cases, i.e. using lipase for hydrolysis of castor oil and analyzing the product through FTIR spectroscopy. (C) 2015 Elsevier B.V. All rights reserved.Article High Carbohydrate Diet Decreases Microbial Diversity and Increases Il-1β Levels in Mice Colon(Korean Society Food Science & Technology-kosfost, 2024) Ulutas, Mehmet Sefa; Aydin, Erkin; Cebeci, AysunWestern diet is known to contribute to intestinal dysbiosis and the progression of inflammation. Although the Turkish diet has different macronutrient contents, the intestinal inflammatory disease incidences in T & uuml;rkiye are comparable to Western countries. Thus, we hypothesized that high carbohydrate diets also contribute to inflammation of the colon. We compared diets with different macronutrient compositions and investigated their effects on colonic microbiota, cytokine, histology, and tight junction protein levels. High carbohydrate diet caused the lowest microbial diversity and is accompanied by the highest expression of interleukin-1 beta and claudin-1. A low carbohydrate diet with zero fiber resulted in the lowest inflammatory markers as well as the lowest occludin and claudin levels. Overall, our results indicate that carbohydrate and fiber contents of the diets are important contributors to colon health.Article Citation - WoS: 4Citation - Scopus: 6One-Pot Conjugated Linoleic Acid Production From Castor Oil by Rhizopus Oryzae Lipase and Resting Cells of Lactobacillus Plantarum(Taylor & Francis Ltd, 2017) Khaskheli, Abid Ali; Talpur, Farah Naz; Aydin, Aysun Cebeci; Jawaid, Sana; Surhio, Muhammad Ali; Afridi, Hassan ImranConjugated linoleic acid (CLA) has attracted as novel type of fatty acids having unusual health-promoting properties such as anticarcinogenic and antiobesitic effects. The present work employed castor oil as substrate for one-pot production of CLA using washed cells of Lactobacillus plantarum (L. plantarum) and lipases as catalysts. Among the screened lipases, the lipase Rhizopus oryzae (ROL) greatly assisted resting cells to produce CLA. Mass spectral analysis of the product showed that two major isomers of CLA were produced in the reaction mixture i.e. cis-9, trans-11 56.55% and trans-10, cis-12 43.45%. Optimum factors for CLA synthesis were found as substrate concentration (8mg/mL), pH (6.5), washed cell concentration (12% w/v), and incubation time of 20h. Hence, the combination of ROL with L. plantarum offers one pot production of CLA selectively using castor oil as a cost-effective substrate.Article Citation - WoS: 35Citation - Scopus: 36Glucose-Dependent Anaplerosis in Cancer Cells Is Required for Cellular Redox Balance in the Absence of Glutamine(Nature Portfolio, 2016) Cetinbas, Naniye Malli; Sudderth, Jessica; Harris, Robert C.; Cebeci, Aysun; Negri, Gian L.; Yilmaz, Oemer H.; Sorensen, Poul H.Cancer cells have altered metabolism compared to normal cells, including dependence on glutamine (GLN) for survival, known as GLN addiction. However, some cancer cell lines do not require GLN for survival and the basis for this discrepancy is not well understood. GLN is a precursor for antioxidants such as glutathione (GSH) and NADPH, and GLN deprivation is therefore predicted to deplete antioxidants and increase reactive oxygen species (ROS). Using diverse human cancer cell lines we show that this occurs only in cells that rely on GLN for survival. Thus, the preference for GLN as a dominant antioxidant source defines GLN addiction. We show that despite increased glucose uptake, GLN addicted cells do not metabolize glucose via the TCA cycle when GLN is depleted, as revealed by C-13-glucose labeling. In contrast, GLN independent cells can compensate by diverting glucose-derived pyruvate into the TCA cycle. GLN addicted cells exhibit reduced PDH activity, increased PDK1 expression, and PDK inhibition partially rescues GLN starvation-induced ROS and cell death. Finally, we show that combining GLN starvation with pro-oxidants selectively kills GLN addicted cells. These data highlight a major role for GLN in maintaining redox balance in cancer cells that lack glucose-dependent anaplerosis.Article Citation - WoS: 12Citation - Scopus: 14Prevention of Cisplatin-Induced Nephrotoxicity by Kidney-Targeted siRNA Delivery(Elsevier, 2022) Aydin, Erkin; Cebeci, Aysun; Lekesizcan, AycaCisplatin is a potent and widely used chemotherapy agent, however, nephrotoxicity limits its use. Many patients need to pause or withdraw from chemotherapy to prevent acute kidney injury. To prevent cisplatin damage, we designed chitosan/siRNA nanoparticleswhich are nontoxic and are readily taken up by HEK293 cells. The nanoparticles contained siRNA against cationic membrane transport (OCT1&2) and apoptosis related proteins (p53, PKC8, and gamma GT). In mice treated with cisplatin, serum creatinine levels increased from 15 to 88 mg/dL and blood urea nitrogen levels increased from 0.25 to 1.7 mg/dL, however, siRNA nanoparticles significantly limited these levels to 30 mg/dL and 0.55 mg/dL, respectively. Western and IHC analyses showed lower p53, PKC8, and gamma GT expressions in siRNA treated mice. Histomorphological evaluation revealed high-level protection of kidney proximal tubules from cisplatin damage. Protein expressions and extent of kidney protection were directly correlated with number of siRNA applications. Our results suggest that this novel approach for kidney -targeted delivery of select siRNAs may represent a promising therapy for preventing cisplatin-induced nephro-toxicity. Furthermore, this or other similarly sized nanocarriers could potentially be utilized to passively target kidneys for diagnostic, protective, or treatment purposes.Article Macronutrients in Diets Differentially Affect Gastrointestinal Cytokine and Tight Junction Protein Levels(Univ Putra Malaysia Press, 2025) Ulutas, M. S.; Cebeci, A.Western diet is known to result in intestinal inflammation and loss of barrier function. In the present work, we investigated whether other macronutrients contribute to inflammation and destabilising barrier function in mice, without using any inflammatory agents, to see the sole effects of dietary intervention. The present work was designed to determine the direct effects of diet on the intestinal barrier function and inflammation, using eight diets that differed on carbohydrate, fat, and fibre ratios for 17 weeks. At the end of the study, a distinct difference in mRNA expressions of cytokines and tight junction proteins was observed between intestinal and colon samples. Small intestinal cytokine expressions showed no difference among different diets, and tight junction protein expressions were only significant for occludin and ZO-1 in high carbohydrate diets. Colon samples had significantly different TNF alpha and IL-6 expressions among diets, especially in high carbohydrate diets. Tight junction protein expressions also differed significantly among diets, and low carbohydrate zero fibre diet had the lowest expression levels compared to the rest of diets. The present work reveals that not only western diet, but also diets high in carbohydrate negatively affect intestinal health, resulting in significant changes in inflammation markers. The role of carbohydrate and fiber contents are also observed in regulating tight junction protein expression. Based on these findings, adjusting macronutrient ratios can be used as a potential approach to help manage intestinal inflammation, though further research is needed. (c) All Rights ReservedArticle Citation - WoS: 3Citation - Scopus: 3Highly Potent New Probiotic Strains From Traditional Turkish Fermented Foods(Springer, 2025) Yigit, Mehmet Burak; Cebeci, AysunTraditional Turkish fermented foods like boza, pickles, and tarhana are recognized for their nutritional and health benefits, yet the probiotic potential of lactic acid bacteria (LAB) strains isolated from them remains underexplored. Sixty-six LAB strains were isolated from fermented foods using bacterial morphology, Gram staining, and catalase activity. The isolates were differentiated at strain level by RAPD-PCR (Random Amplification of Polymorphic DNA-Polymerase Chain Reaction) and twenty-five strains were selected for further evaluation of acid and bile salt tolerance. Among these, ten strains exhibited high tolerance and were subsequently assessed for adhesion to Caco-2 colorectal carcinoma cells, antimicrobial activity, exopolysaccharide (EPS) production, lysozyme resistance, and hemolytic activity. Using k-means clustering, three strains: Lactiplantibacillus plantarum ES-3, Pediococcus pentosaceus N-1, and Enterococcus faecium N-2 demonstrated superior probiotic characteristics, including significant acid (100% survival at pH3.0) and 0.3% bile salt tolerance (57%, 64%, 67%), strong adhesion to intestinal cells (65%, 88%, 91%), high lysozyme resistance (88%, 88%, 77%), and produced high amounts of EPS. These strains show promising potential as probiotics and warrant further investigation to confirm their functional properties and potential applications.Article Bazı geleneksel Türk gıdalarından laktik asit bakterilerinin izolasyonu(2021) DOĞAN Osman; CEBECİ AysunAmaç: Bu çalışma ülkemizde geleneksel yöntemlerle üretilen gıda ürünlerindenlaktik asit bakterilerinin izolasyonunu ve tanımlanmasını sağlamak amacıylayapılmıştır.Materyal ve Yöntem: Çalışma kapsamında Van otlu peynir ve ekşi hamur örneğikullanılmıştır. Bu örnekler içerdikleri laktik asit bakterileri için çalışılmış vetanımlanmaları için biyokimyasal ve PCR bazlı moleküler biyolojik tekniklere tabitutulmuşlardır. Biyokimyasal testler kapsamında örnekler, Gram reaksiyonları,katalaz aktivitesi, gaz üretimi, 10oC ve 45oC'de, %6 ve %16 NaCl konsantrasyonda,pH 4.4 ve pH 9.6’da gelişim göstermeleri açısından incelenmiştir. Moleküler biyolojideneyleri kapsamında ise tür ve suş düzeyinde tanımlama için PCR-RFLP, 16SrRNA gen dizileme ve RAPD-PCR teknikleri kullanılmıştır.Araştırma Bulguları: Bir dizi mikrobiyolojik deneylerin sonucunda 26 adet bakteripotansiyel laktik asit bakterisi olarak izole edilmiştir. Bunlardan 25 adedininLactobacillus, Pediococcus ve Enterococcus cinslerine ait olduğu tespit edilmiş vetür ve suş düzeyinde tanımlanmaları sağlanmıştır. Kalan bir adet izolat iseStaphylococcus hominis olarak tanımlanmıştır.Sonuç: Çalışmamız sonucunda 25 adet laktik asit bakterisi gen dizileme ve RAPDPCR teknikleri kullanılarak tür ve suş düzeyinde başarıyla tanımlanmıştır.Article Tarhanadan Laktik Asit Bakterilerinin İzolasyonu(2020) Aydın, Aysun Cebeci; Çalış, Burak; Polat, Muhammet FatihTarhana is a staple food in Turkey and produced mainly by the fermentation of wheat flour and yogurt.There are variations in its use, it can be consumed as a snack, or as a soup, and its exact ingredients vary betweendifferent geographical regions. Our study aims to isolate and identify lactic acid bacteria in tarhana samples viabiochemical and molecular biology identification techniques. Three different tarhana samples were studied forbacterial Gram reactions, catalase activity, gas production, growth at 10°C and 45°C, in 6% and 16% NaClconcentrations and at pH 4,4 and pH 9,6 for the biochemical tests. For the molecular biology experiments, PCRRFLP, sequencing and RAPD-PCR were performed to identify organisms at the species and strain level.Master Thesis Geleneksel Gıda Ürünlerinden İzole Edilen Laktik Asit Bakterilerinin Probiyotik Özelliklerinin Belirlenmesi(Abdullah Gül Üniversitesi, Fen Bilimleri Enstitüsü, 2023) Yiğit, Mehmet Burak; Aydın, Aysun CebeciProbiotics are microorganisms that live in our bodies and positively affect health when consumed regularly. One of the ways to have a healthy body is to have a healthy microbiota. Because of that, the importance given to the consumption of probiotic foods among the public is increasing. Since probiotics are especially abundant in fermented and traditional foods, consuming these foods is vital to have a healthy microflora. In this thesis, probiotic potentials of bacteria isolated from tarhana, einkorn sourdough, Turkish and Bulgarian-type boza and pickled beetroot foods were investigated, and obtained results were discussed. Based on acid and bile salt tolerance tests, MRS ES-2-3-7-11-12-17, MRS PT-2-14-16, MRS N-1, MRS EB-3, MRS T-1, M17 N-2 -3-4 showed higher viability in acidic environments (pH 2.0 and 3.0) than the control groups, M17 N-3-4 and M17 TB-1-2 strains showed higher viability at 0.3% and 0.5% bile salt conditions than other strains. For 10 strains which are selected for further tests, in the adhesion to Caco-2 cells, MRS ES-3, MRS N-1, MRS T-2, M17 BB-7, M17 N-2 and M17 N-3 showed over 35% adhesion, especially, MRS N-1 and M17 N-2 showed over 85% adhesion to Caco-2 cells. For the antimicrobial activity test, ES-3 strain showed limited effect on S. aureus ATCC 6538 and K. pneumoniae ATCC 4352 pathogens, while other strains showed no inhibitory effect on pathogens. Finally, according to the results of 16S rRNA sequencing, it was determined that MRS ES-3, ES-7, PT-14 strains belonged to L. plantarum, MRS ES-11 strain belonged to L. brevis, M17 BB-7 strain belonged to E. faecium and M17 TB-2 strain belonged to E. durans species.
