Biçer Çalışkan, Mesude
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Bicer, Mesude
M Bicer-Çalışkan
M Bicer-Çalışkan
Job Title
Dr. Öğr. Üyesi
Email Address
mesude.bicer@agu.edu.tr
Main Affiliation
04.01. Biyomühendislik
Status
Current Staff
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Sustainable Development Goals
3
GOOD HEALTH AND WELL-BEING
2
Research Products
5
GENDER EQUALITY
1
Research Products
Documents
8
Citations
119
h-index
5
Documents
9
Citations
109
Scholarly Output
7
Articles
7
Views / Downloads
4/0
Supervised MSc Theses
0
Supervised PhD Theses
0
WoS Citation Count
25
Scopus Citation Count
30
WoS h-index
3
Scopus h-index
3
Patents
0
Projects
0
WoS Citations per Publication
3.57
Scopus Citations per Publication
4.29
Open Access Source
6
Supervised Theses
0
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| Journal | Count |
|---|---|
| ACS Infectious Diseases | 1 |
| Archives of Dermatological Research | 1 |
| Archives of Microbiology | 1 |
| Biomolecules | 1 |
| Celal Bayar Üniversitesi Fen Bilimleri Dergisi | 1 |
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Scholarly Output Search Results
Now showing 1 - 7 of 7
Article Citation - WoS: 10Citation - Scopus: 14Revolutionizing Dermatology: Harnessing Mesenchymal Stem/Stromal Cells and Exosomes in 3D Platform for Skin Regeneration(Springer, 2024) Bicer, MesudeContemporary trends reveal an escalating interest in regenerative medicine-based interventions for addressing refractory skin defects. Conventional wound healing treatments, characterized by high costs and limited efficacy, necessitate a more efficient therapeutic paradigm to alleviate the economic and psychological burdens associated with chronic wounds. Mesenchymal stem/stromal cells (MSCs) constitute cell-based therapies, whereas cell-free approaches predominantly involve the utilization of MSC-derived extracellular vesicles or exosomes, both purportedly safe and effective. Exploiting the impact of MSCs by paracrine signaling, exosomes have emerged as a novel avenue capable of positively impacting wound healing and skin regeneration. MSC-exosomes confer several advantages, including the facilitation of angiogenesis, augmentation of cell proliferation, elevation of collagen production, and enhancement of tissue regenerative capacity. Despite these merits, challenges persist in clinical applications due to issues such as poor targeting and facile removal of MSC-derived exosomes from skin wounds. Addressing these concerns, a three-dimensional (3D) platform has been implemented to emend exosomes, allowing for elevated levels, and constructing more stable granules possessing distinct therapeutic capabilities. Incorporating biomaterials to encapsulate MSC-exosomes emerges as a favorable approach, concentrating doses, achieving intended therapeutic effectiveness, and ensuring continual release. While the therapeutic potential of MSC-exosomes in skin repair is broadly recognized, their application with 3D biomaterial scenarios remains underexplored. This review synthesizes the therapeutic purposes of MSCs and exosomes in 3D for the skin restoration, underscoring their promising role in diverse dermatological conditions. Further research may establish MSCs and their exosomes in 3D as a viable therapeutic option for various skin conditions.Article Citation - WoS: 3Citation - Scopus: 4Can Mesenchymal Stem/Stromal Cells and Their Secretomes Combat Bacterial Persisters(Springer, 2023) Bicer, Mesude; Fidan, OzkanThe increasing number of life-threatening infections caused by persister bacteria is associated with various issues, including antimicrobial resistance and biofilm formation. Infections due to persister cells are often difficult to suppress without the use of last-resort antibiotics. Throughout the world, bacterial persistence and resistance create an unmet clinical demand for the exploration of newly introduced therapeutic approaches. Mesenchymal stem / stromal cells (MSCs) have an antimicrobial activity to protect against bacterial infections, including those caused by bacterial persisters. MSCs have substantial potential to secrete antimicrobial peptides (AMPs), including cathelicidin, beta-defensins, lipocalin-2, hepcidin, indoleamine 2,3-dioxygenase (IDO), cysteine proteases, and inducible nitric oxide synthases (iNOS). MSCs possess the potential to contribute to innate immunity by regulating the immune response. Recently, MSCs and their secreted components have been reported to improve antimicrobial activity. Bactericidal activity by MSCs and their secretomes has been shown to be mediated in part by the secretion of AMPs. Even though they were discovered more than 80 years ago, therapeutic options for persisters are restricted, and there is an urgent need for alternative treatment regimens. Hence, this review intends to critically assess the current literature on the effects of MSCs and their secretomes on persister bacteria. MSCs and their secretome-based therapies could be preferred as an up-and-coming approach to reinforce the antimicrobial efficiency in persister infections.Article Candida Enfeksiyonlarına Karşı Toll-Benzeri Reseptörlerin ve Antimikrobiyal Peptitlerin Özelleştirilmesine Yönelik Hesaplamalı Yaklaşımlardaki Son Gelişmeler(2025) Bicer, Mesude; Serçinoğlu, Onur; Okur, TubaCandida albicans'ın insan sağlığı üzerindeki kayda değer patojenik etkisine rağmen, hücresel tanıma mekanizmalarının ve ardından gelen konakçı savunma aktivasyonunun anlaşılmasındaki boşluk yeterince anlaşılmamıştır. Son bilgiler, Toll benzeri reseptörlerin (TLR'ler) patojenlere karşı doğuştan gelen bağışıklık tepkilerini düzenlemedeki önemli rolünün altını çiziyor. Özellikle, son yıllardaki ampirik araştırmalar, TLR'lerin memelilerde en önemli model tanıma reseptörleri olduğunun altını çizmiştir. Örneğin TLR2, peptidoglikanlar, lipoarabinomannan ve bakteriyel lipoproteinler için afinite sergilerken TLR4, lipopolisakkarit (LPS) ve lipo-teikoik asidin saptanmasında rol oynar. Benzer şekilde TLR5 flagellini tanır ve TLR9 bakteriyel DNA tanımayla ilişkilidir. Toll'un Drosophila'da antifungal mekanizmaların düzenleyicisi olarak ilk tanımlanması, TLR'lerin memeli antifungal savunmasında potansiyel olarak dahil olduğunu düşündürmektedir. Bununla birlikte, Drosophila'daki Toll ile antifungal mekanizmalar arasındaki evrimsel bağlantıya rağmen, insanlarda fungal patojenlerle mücadelede TLR'lerin rolünün tanımlanmasına çok az önem verilmiştir; bu, TLR'lerin memeli antifungal savunmasında makul bir rol oynadığını düşündürmektedir. Özellikle kanıtlar, Aspergillus fumigatus'a yanıt olarak proinflamatuar sitokinleri indüklemede TLR4'ü gösterir ancak TLR2'yi kapsamaz; bu arada rolünün, hücrelerin Cryptococcus neoformans ile uyarılmasından sonra TNF üretimi olmasa da hücre içi sinyalleşmeye aracılık ettiği iddia edilir. Bununla birlikte, TLR aktivasyon kurallarına ilişkin içgörüler, antimikrobiyal peptit (AMP) ile TLR etkileşimlerinin incelenmesini mümkün kılmaktadır ve çeşitli moleküllerin immünomodülatör kapasitelerine ilişkin tahminleri kolaylaştırmaktadırç Bu ilerlemelere rağmen, TLR'lerin önde gelen bir insan patojeni olan Candida albicans'ı tanımadaki spesifik rolü hala belirsizliğini koruyor ve daha fazla araştırma yapılmasını gerektiriyor. Bu hesaplamalı yaklaşım, AMP'ler ve TLR'ler arasındaki etkileşimleri aydınlatan, TLR aktivasyonunu yöneten yapısal belirleyicileri tanımlayan ve böylece çeşitli moleküler varlıkların immünomodülatör potansiyeline ilişkin öngörüler sağlayan son bulguları sentezlemektedir.Article Assessing the Proliferative Impact of Alginate-Gelatin Composites on Mesenchymal Stromal Cells(2025) Bicer, Mesudeİki boyutlu (2D) kültür üzerinde yapılan geleneksel çalışmalar, hücre göçü, çözünür faktör gradyanı ve hücre-matris etkileşimleri dahil olmak üzere in vivo ortamın fizyolojik ve patofizyolojik özelliklerini yakından yansıttmaz. 3-boyutlu (3D) hücre kültürü, hücre göçünü teşvik etmek, biyomateryal sertligini degiştirmek veya hücre-matris etkileşimlerine izin vermek için aljinat hidrojelleri gibi 3D biyomateryalleri kullanarak bu dezavantajların üstesinden gelmektedir. Bu çalışmada, mezenkimal kok hücre canlılığını ve farklılaşma potansiyelini destekleyen doku mühendisliği tekniklerine olan ihtiyacı karşılamak için aljinat-jelatin kompozitleri içeren yeni bir 3-boyutlu platformun önerilmesi amaçlanmıştır. İlk bölümde farklı hidrojel bazlı biyomateryallerin absorbans spektrumları görünür ışık kullanılarak incelenmiştir. En iyi performansı gösteren hidrojeli bulduktan sonra çalışma, XTT canlılık testi ve canlı/ölü sitotoksisite testi kullanılarak hücre çoğalmasına odaklanılmıştır. Hücre canlılığı aljinat-jelatin, aljinat ve selüloz gibi iskelelerle karşılaştırıldığında, aljinat-jelatin hidrojelin mezenkimal kök hücrelerinin canlılığını arttırdığı tespit edildi. Bu artış, 2D kültürde büyütülen hücrelerle karşılaştırıldığında da aynı şekilde gözlendi. Bu çalışmadan elde edilen bulgular, literatürdeki diğer çalışmaların verileriyle tutarlıdır. Bu nedenle, aljinat-jelatin kompozitleri doku mühendisliğinde hücre proliferasyonunu iyileştirmek için umut verici bir aday olabilir.Article Citation - WoS: 1Antifungal Efficacy of 3D-Cultured Palatal Mesenchymal Stem Cells and Their Secreted Factors Against Candida albicans(American Chemical Society, 2025) Bicer, M.; Öztürk, E.; Sener, F.; Hakki, S.S.; Fidan, O.Candida albicans is among the life-threatening fungal species and the primary contributor to hospital-acquired systemic infections, accounting for nearly 70% of all fungal infections worldwide. The current treatment primarily relies on azoles, pyrimidine analogs, polyenes, and echinocandins. However, growing antifungal resistance highlights the urgent need for the development of alternative treatments against C. albicans. Mesenchymal stem cells (MSCs) offer huge therapeutic potential for the treatment of C. albicans-associated diseases. In this study, palatal adipose tissue-derived MSCs (PAT-MSCs) and PAT-MSCs cultured in 3D biomaterial using nanofibrillar cellulose were tested against C. albicans strains ATCC 10231 and ATCC MYA 2876 using an in vitro antifungal activity assay. In addition, the conditioned medium from both PAT-MSCs and PAT-MSCs cultured in 3D hydrogel biomaterial (CM-PAT-MSCs-3D) were evaluated for their antifungal activities. The combined effect of PAT-MSCs and their secreted factors was also investigated. The expression of five antimicrobial peptide (AMP)-encoding genes was analyzed by quantitative real-time PCR. The expression of antimicrobial peptides was further confirmed via immunocytochemical staining. PAT-MSCs significantly inhibited the growth of C. albicans strains at varying inoculum concentrations (500 and 2000 CFU). Similarly, a comparable antifungal effect was observed when Candida strains were treated with PAT-MSC secreted factors alone. Statistical analysis revealed significant differences between the antifungal activities of PAT-MSCs and CM-PAT-MSCs. Lastly, the combination of PAT-MSCs and CM-PAT-MSC-3D led to a marked reduction in fungal growth, with inhibition rates of 99.75% and 99.91% for C. albicans ATCC 10231 and ATCC MYA-2876, respectively, at 500 CFU inocula. At 2000 CFU inocula, inhibition rates were 99.54% and 99.91%, respectively (****P ≤ 0.0001). These antifungal activities were further confirmed by using RT-PCR and immunocytochemical analysis. Our findings underscore a perspective on the potent antifungal activity of secreted factors from PAT-MSCs cultured within a 3D hydrogel matrix, specifically against various strains of C. albicans. Particularly, the combination of PAT-MSCs with their secreted factors represents a promising therapeutic platform, potentially offering a safer and more effective alternative to conventional antifungal treatments. © 2025 Elsevier B.V., All rights reserved.Article Citation - WoS: 3Citation - Scopus: 3Exploring Therapeutic Avenues: Mesenchymal Stem/Stromal Cells and Exosomes in Confronting Enigmatic Biofilm-Producing Fungi(Springer, 2024) Bicer, MesudeFungal infections concomitant with biofilms can demonstrate an elevated capacity to withstand substantially higher concentrations of antifungal agents, contrasted with infectious diseases caused by planktonic cells. This inherent resilience intrinsic to biofilm-associated infections engenders a formidable impediment to effective therapeutic interventions. The different mechanisms that are associated with the intrinsic resistance of Candida species encompass drug sequestration by the matrix, drug efflux pumps, stress response cell density, and the presence of persister cells. These persisters, a subset of fungi capable of surviving hostile conditions, pose a remarkable challenge in clinical settings in virtue of their resistance to conventional antifungal therapies. Hence, an exigent imperative has arisen for the development of novel antifungal therapeutics with specific targeting capabilities focused on these pathogenic persisters. On a global scale, fungal persistence and their resistance within biofilms generate an urgent clinical need for investigating recently introduced therapeutic strategies. This review delves into the unique characteristics of Mesenchymal stem/stromal cells (MSCs) and their secreted exosomes, which notably exhibit immunomodulatory and regenerative properties. By comprehensively assessing the current literature and ongoing research in this field, this review sheds light on the plausible mechanisms by which MSCs and their exosomes can be harnessed to selectively target fungal persisters. Additionally, prospective approaches in the use of cell-based therapeutic modalities are examined, emphasizing the importance of further research to overcome the enigmatic fungal persistence.Article Citation - WoS: 8Citation - Scopus: 9Time-Dependent Reduction of Calcium Oscillations in Adipose-Derived Stem Cells Differentiating Towards Adipogenic and Osteogenic Lineage(MDPI, 2021) Torre, Enrico C.; Bicer, Mesude; Cottrell, Graeme S.; Widera, Darius; Tamagnini, FrancescoAdipose-derived mesenchymal stromal cells (ASCs) are multipotent stem cells which can differentiate into various cell types, including osteocytes and adipocytes. Due to their ease of harvesting, multipotency, and low tumorigenicity, they are a prime candidate for the development of novel interventional approaches in regenerative medicine. ASCs exhibit slow, spontaneous Ca2+ oscillations and the manipulation of Ca2+ signalling via electrical stimulation was proposed as a potential route for promoting their differentiation in vivo. However, the effects of differentiation-inducing treatments on spontaneous Ca2+ oscillations in ASCs are not yet fully characterised. In this study, we used 2-photon live Ca2+ imaging to assess the fraction of cells showing spontaneous oscillations and the frequency of the oscillation (measured as interpeak interval-IPI) in ASCs undergoing osteogenic or adipogenic differentiation, using undifferentiated ASCs as controls. The measurements were carried out at 7, 14, and 21 days in vitro (DIV) to assess the effect of time in culture on Ca2+ dynamics. We observed that both time and differentiation treatment are important factors associated with a reduced fraction of cells showing Ca2+ oscillations, paralleled by increased IPI times, in comparison with untreated ASCs. Both adipogenic and osteogenic differentiation resulted in a reduction in Ca2+ dynamics, such as the fraction of cells showing intracellular Ca2+ oscillations and their frequency. Adipogenic differentiation was associated with a more pronounced reduction of Ca2+ dynamics compared to cells differentiating towards the osteogenic fate. Changes in Ca2+ associated oscillations with a specific treatment had already occurred at 7 DIV. Finally, we observed a reduction in Ca2+ dynamics over time in untreated ASCs. These data suggest that adipogenic and osteogenic differentiation cell fates are associated with specific changes in spontaneous Ca2+ dynamics over time. While this observation is interesting and provides useful information to understand the functional correlates of stem cell differentiation, further studies are required to clarify the molecular and mechanistic correlates of these changes. This will allow us to better understand the causal relationship between Ca2+ dynamics and differentiation, potentially leading to the development of novel, more effective interventions for both bone regeneration and control of adipose growth.
