Scopus İndeksli Yayınlar Koleksiyonu
Permanent URI for this collectionhttps://hdl.handle.net/20.500.12573/395
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Article Borax-Doped Fe2O3 and CeO2 Nanoparticles Regulate Dose-Dependently Inflammation, the Cell Cycle, and Migration in LPS-Activated THP-1 Cells(Wiley-VCH Verlag GmbH, 2026-03) Sulak, Mine; Ceylan Ekiz, Yağmur; Şen, Alaattin; Acar, Büşra; Çelik Turgut, Gurbet; Aktaş Pepe, NihanThis study examined the biological effects of borax-doped Fe2O3 and CeO2 nanoparticles (NPs) on lipopolysaccharide (LPS)-activated THP-1 cells. The morphology and composition of the nanocomposites were confirmed via scanning electron microscopy (SEM) and energy-dispersive x-ray spectroscopy (EDX). Cell viability (resazurin and crystal violet assays), apoptosis/necrosis (annexin V/propidium iodide [PI]), cell cycle (flow cytometry), migration (scratch assay), and inflammatory response (Iba1 immunofluorescence staining, inducible nitric oxide synthase [iNOS] activity, and RT-PCR) were evaluated. The particle sizes ranged from 21.34 to 33.47 nm (Fe2O3-B-NPs) and 31.07 to 36.62 nm (CeO2-B-NPs). The IC10 and IC50 dose ranges were defined for each nanocomposite and applied across different cell lines to evaluate dose-dependent biological effects. Fe2O3-B-NPs altered cell cycle progression, increasing the number of S phase cells. Both nanocomposites promoted migration at low doses but inhibited it at high doses. CeO2-B-NPs reduced Iba1 levels, whereas Fe2O3-B-NPs increased inflammatory marker levels at higher concentrations. CeO2-B-NPs suppressed TNF-alpha and IL-1 beta gene expression at the IC50 dose, while both nanocomposites reduced iNOS activity. These results indicate that the dose-dependent effects of nanocomposites should be carefully evaluated.Article Macronutrients in Diets Differentially Affect Gastrointestinal Cytokine and Tight Junction Protein Levels(Univ Putra Malaysia Press, 2025-02-01) Ulutas, M. S.; Cebeci, A.Western diet is known to result in intestinal inflammation and loss of barrier function. In the present work, we investigated whether other macronutrients contribute to inflammation and destabilising barrier function in mice, without using any inflammatory agents, to see the sole effects of dietary intervention. The present work was designed to determine the direct effects of diet on the intestinal barrier function and inflammation, using eight diets that differed on carbohydrate, fat, and fibre ratios for 17 weeks. At the end of the study, a distinct difference in mRNA expressions of cytokines and tight junction proteins was observed between intestinal and colon samples. Small intestinal cytokine expressions showed no difference among different diets, and tight junction protein expressions were only significant for occludin and ZO-1 in high carbohydrate diets. Colon samples had significantly different TNF alpha and IL-6 expressions among diets, especially in high carbohydrate diets. Tight junction protein expressions also differed significantly among diets, and low carbohydrate zero fibre diet had the lowest expression levels compared to the rest of diets. The present work reveals that not only western diet, but also diets high in carbohydrate negatively affect intestinal health, resulting in significant changes in inflammation markers. The role of carbohydrate and fiber contents are also observed in regulating tight junction protein expression. Based on these findings, adjusting macronutrient ratios can be used as a potential approach to help manage intestinal inflammation, though further research is needed. (c) All Rights Reserved