Scopus İndeksli Yayınlar Koleksiyonu
Permanent URI for this collectionhttps://hdl.handle.net/20.500.12573/395
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Article Electrospun PCL/PEG Nanofibers Incorporating Plantago Lanceolata Extract and Clove Oil for Dual-Function Wound Dressings(IOP Publishing Ltd, 2026) Teke, Selin Nur; Yuruk, Adile; Isoglu, Ismail AlperIn this study, we developed electrospun polycaprolactone/polyethylene glycol (PCL/PEG) nanofibers loaded with Plantago lanceolata (P. lanceolata) extract and clove oil to evaluate their combined potential for wound healing and antibacterial activity. PCL/PEG nanofibers were electrospun and post-loaded with P. lanceolata extract at 5%, 10%, and 15% (w/v), together with 1% (v/v) clove oil. Scanning electron microscope analysis showed a uniform, bead-free nanofibrous structure, with fiber diameters ranging from 768 +/- 140 nm to 892 +/- 206 nm and pore sizes from 3.93 +/- 0.97 & micro;m to 6.20 +/- 1.16 & micro;m. The nanofibers exhibited swelling ratios between 103.23 +/- 16.42% and 133.93 +/- 40.45% within 1 h and showed gradual degradation ranging from 25.92 +/- 2.84% to 57.76 +/- 0.92% over 21 d, with cumulative extract release approaching a plateau by day 28 under the experimental conditions. The incorporation of plant extract and essential oil initially increased the water contact angle from 15.97 +/- 1.07 degrees to 48.61 +/- 7.85 degrees, indicating that the nanofiber surface remained hydrophilic; at higher extract contents, the nanofibers transitioned to a superhydrophilic state. Antibacterial activity was primarily governed by clove oil, yielding up to 85.16 +/- 0.36% efficacy against E. coli (E. coli) and 79.90 +/- 0.29% against S. aureus (S. aureus). While P. lanceolata extract alone showed limited antibacterial activity, its presence within clove oil-loaded nanofibers consistently enhanced antibacterial performance at the composite level. In vitro scratch assays demonstrated pronounced wound closure at later time points (72-96 h), particularly for nanofibers containing higher extract concentrations, confirming the dominant contribution of P. lanceolata to the healing response. Overall, the electrospun PCL/PEG nanofibers represent a dual-function wound dressing, with clove oil contributing antibacterial protection and P. lanceolata extract supporting wound healing.Article Citation - WoS: 9Citation - Scopus: 12Stem Cells Combined 3D Electrospun Nanofibrous and Macrochannelled Matrices: A Preliminary Approach in Repair of Rat Cranial Bones(Taylor & Francis Ltd, 2019-04-03) Isoglu, Ismail Alper; Bolgen, Nimet; Korkusuz, Petek; Vargel, Ibrahim; Celik, Hakan Hamdi; Kilic, Emine; Piskin, ErhanRepair of cranial bone defects is an important problem in the clinical area. The use of scaffolds combined with stem cells has become a focus in the reconstruction of critical-sized bone defects. Electrospinning became a very attracting method in the preparation of tissue engineering scaffolds in the last decade, due to the unique nanofibrous structure of the electrospun matrices. However, they have a limitation for three dimensional (3D) applications, due to their two-dimensional structure and pore size which is smaller than a cellular diameter which cannot allow cell migration within the structure. In this study, electrospun poly(epsilon-caprolactone) (PCL) membranes were spirally wounded to prepare 3D matrices composed of nanofibers and macrochannels. Mesenchymal stromal/stem cells were injected inside the scaffolds after the constructs were implanted in the cranial bone defects in rats. New bone formation, vascularisation and intramembranous ossification of the critical size calvarial defect were accelerated by using mesenchymal stem cells combined 3D spiral-wounded electrospun matrices.