Scopus İndeksli Yayınlar Koleksiyonu
Permanent URI for this collectionhttps://hdl.handle.net/20.500.12573/395
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Article Electrospun PCL/PEG Nanofibers Incorporating Plantago Lanceolata Extract and Clove Oil for Dual-Function Wound Dressings(IOP Publishing Ltd, 2026) Teke, Selin Nur; Yuruk, Adile; Isoglu, Ismail AlperIn this study, we developed electrospun polycaprolactone/polyethylene glycol (PCL/PEG) nanofibers loaded with Plantago lanceolata (P. lanceolata) extract and clove oil to evaluate their combined potential for wound healing and antibacterial activity. PCL/PEG nanofibers were electrospun and post-loaded with P. lanceolata extract at 5%, 10%, and 15% (w/v), together with 1% (v/v) clove oil. Scanning electron microscope analysis showed a uniform, bead-free nanofibrous structure, with fiber diameters ranging from 768 +/- 140 nm to 892 +/- 206 nm and pore sizes from 3.93 +/- 0.97 & micro;m to 6.20 +/- 1.16 & micro;m. The nanofibers exhibited swelling ratios between 103.23 +/- 16.42% and 133.93 +/- 40.45% within 1 h and showed gradual degradation ranging from 25.92 +/- 2.84% to 57.76 +/- 0.92% over 21 d, with cumulative extract release approaching a plateau by day 28 under the experimental conditions. The incorporation of plant extract and essential oil initially increased the water contact angle from 15.97 +/- 1.07 degrees to 48.61 +/- 7.85 degrees, indicating that the nanofiber surface remained hydrophilic; at higher extract contents, the nanofibers transitioned to a superhydrophilic state. Antibacterial activity was primarily governed by clove oil, yielding up to 85.16 +/- 0.36% efficacy against E. coli (E. coli) and 79.90 +/- 0.29% against S. aureus (S. aureus). While P. lanceolata extract alone showed limited antibacterial activity, its presence within clove oil-loaded nanofibers consistently enhanced antibacterial performance at the composite level. In vitro scratch assays demonstrated pronounced wound closure at later time points (72-96 h), particularly for nanofibers containing higher extract concentrations, confirming the dominant contribution of P. lanceolata to the healing response. Overall, the electrospun PCL/PEG nanofibers represent a dual-function wound dressing, with clove oil contributing antibacterial protection and P. lanceolata extract supporting wound healing.Article Citation - Scopus: 1Engineering a Bilayered Scaffold as a Potential Cardiac Patch: From Scaffold Design to in Vitro Assessment(Springer Singapore Pte Ltd, 2025-11-24) Yuruk, Adile; Duzler, Ayhan; Isoglu, Sevil Dincer; Isoglu, Ismail AlperIn this study, we developed a novel bilayered scaffold consisting of a bottom layer composed of the Decellularized Bovine Pericardium (DP) coated with Polyaniline Nanoparticles (PANINPs) and a top layer made of an electrospun Poly(lactic-co-glycolic acid)/Gelatin (PLGA/Gel) membrane incorporated with Vascular Endothelial Growth Factor (VEGF) and hawthorn extract. Functionally, the DP supplies native Extracellular Matrix (ECM) components and mechanical support, while PANINPs provide conductivity. The electrospun PLGA/Gel layer mimics fibrous ECM. It incorporates bioactives, with VEGF promoting pro-angiogenic stimulation and hawthorn extract enhancing anticoagulant activity, as well as increasing surface hydrophilicity. The tissue adhesive ensures the interfacial integrity between the two layers. Decellularization efficiency was confirmed histologically using 4 ',6-diamidino-2-phenylindole (DAPI) and Hematoxylin-Eosin (H&E) staining. The DP exhibited a DNA content of 115.9 +/- 47.8 ng/mg DNA, compared to 982.88 +/- 395.42 ng/mg in Native Pericardium (NP). The PANINPs had an average particle size of 104.94 +/- 13.7 nm. The conductivity of PANINPs-coated decellularized pericardium was measured to be 9.093 +/- 8.6 x 10- 4 S/cm using the four-point probe method. PLGA/Gel membranes containing hawthorn extract (1%, 5%, 10%, and 15% w/v) and VEGF (0.1 mu g/mL, 0.5 mu g/mL, and 1 mu g/mL) were fabricated by electrospinning, resulting in fiber diameters between 850 and 1200 nm and pore sizes between 14 and 20 mu m. The anticoagulant efficiency of the membranes containing hawthorn extract reached 430 s in the Activated Partial Thromboplastin Time Assay (aPTT). Mechanical testing revealed a tensile strength of 22.70 +/- 6.33 MPa, an elongation of 53.58 +/- 10.63%, and Young's modulus of 0.67 +/- 0.10 MPa. The scaffold also exhibited over 91% cell viability and excellent cardiomyocyte adhesion. The hemolysis ratio was determined to be 0.421 +/- 0.191%, which confirms its blood compatibility. Our results indicate that the proposed bilayered scaffold can be a promising candidate for cardiac patch applications.