Scopus İndeksli Yayınlar Koleksiyonu
Permanent URI for this collectionhttps://hdl.handle.net/20.500.12573/395
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Article Citation - WoS: 15Citation - Scopus: 16Image-Analysis Based Readout Method for Biochip: Automated Quantification of Immunomagnetic Beads, Micropads and Patient Leukemia Cell(Pergamon-Elsevier Science Ltd, 2020-06) Uslu, Fatma; Icoz, Kutay; Tasdemir, Kasim; Dogan, Refika S.; Yilmaz, BulentFor diagnosing and monitoring the progress of cancer, detection and quantification of tumor cells is utmost important. Beside standard bench top instruments, several biochip-based methods have been developed for this purpose. Our biochip design incorporates micron size immunomagnetic beads together with micropad arrays, thus requires automated detection and quantification of not only cells but also the micropads and the immunomagnetic beads. The main purpose of the biochip is to capture target cells having different antigens simultaneously. In this proposed study, a digital image processing-based method to quantify the leukemia cells, immunomagnetic beads and micropads was developed as a readout method for the biochip. Color, size-based object detection and object segmentation methods were implemented to detect structures in the images acquired from the biochip by a bright field optical microscope. It has been shown that manual counting and flow cytometry results are in good agreement with the developed automated counting. Average precision is 85 % and average error rate is 13 % for all images of patient samples, average precision is 99 % and average error rate is 1% for cell culture images. With the optimized micropad size, proposed method can reach up to 95 % precision rate for patient samples with an execution time of 90 s per image.Article Citation - WoS: 6Citation - Scopus: 7BLSTM Based Night-Time Wildfire Detection From Video(Public Library Science, 2022-06-03) Agirman, Ahmet K.; Tasdemir, KasimDistinguishing fire from non-fire objects in night videos is problematic if only spatial features are to be used. Those features are highly disrupted under low-lit environments because of several factors, such as the dynamic range limitations of the cameras. This makes the analysis of temporal behavior of night-time fire indispensable for classification. To this end, a BLSTM based night-time wildfire event detection from a video algorithm is proposed. It is shown in the experiments that the proposed algorithm attains 95.15% of accuracy when tested against a wide variety of actual recordings of night-time wildfire incidents and 23.7 ms per frame detection time. Moreover, to pave the way for more targeted solutions to this challenging problem, experiment-based thorough investigations of possible sources of incorrect predictions and discussion of the unique nature of night-time wildfire videos are presented in the paper.