Scopus İndeksli Yayınlar Koleksiyonu
Permanent URI for this collectionhttps://hdl.handle.net/20.500.12573/395
Browse
2 results
Search Results
Article High Carbohydrate Diet Decreases Microbial Diversity and Increases Il-1β Levels in Mice Colon(Korean Society Food Science & Technology-kosfost, 2024-05-04) Ulutas, Mehmet Sefa; Aydin, Erkin; Cebeci, AysunWestern diet is known to contribute to intestinal dysbiosis and the progression of inflammation. Although the Turkish diet has different macronutrient contents, the intestinal inflammatory disease incidences in T & uuml;rkiye are comparable to Western countries. Thus, we hypothesized that high carbohydrate diets also contribute to inflammation of the colon. We compared diets with different macronutrient compositions and investigated their effects on colonic microbiota, cytokine, histology, and tight junction protein levels. High carbohydrate diet caused the lowest microbial diversity and is accompanied by the highest expression of interleukin-1 beta and claudin-1. A low carbohydrate diet with zero fiber resulted in the lowest inflammatory markers as well as the lowest occludin and claudin levels. Overall, our results indicate that carbohydrate and fiber contents of the diets are important contributors to colon health.Article Citation - WoS: 9Citation - Scopus: 13A Highly Selective Whole Cell Biocatalysis Method for the Production of Two Major Bioactive Conjugated Linoleic Acid Isomers(Elsevier, 2013-10) Khaskheli, Abid Ali; Talpur, Farah N.; Demir, Ayhan S.; Cebeci, Aysun; Jawaid, SanaConjugated linoleic acid (CLA) is well known potential anticarcinogenic, antiatherogenic and a number of other therapeutic qualities bearing substance. In the present study we have isolated Lactobacillus plantarum from local dairy buffalo milk and used as a whole cell biocatalyst, for selective CLA isomer production from linoleic acid (LA). Quantitative analysis revealed that the cis-9, trans-11 CLA comprised of 51% (w/w), while trans-10, cis-12 CLA accounted for 49% (w/w) of total CLA produced by L. plantarum. The product formation and quantitation was monitored by the spectrophotometric method and the individual isomers separation was done with GCFID. After optimization of biotransformation conditions such as pH, LA concentration, effect of preincubation, fermentation time; the highest CLA production (19.07% conversion) was achieved at 120 h in the presence of 1.62 mg ml-1 of LA. The results revealed that the bacterium is an efficient biocatalyst for the production of two major bioactive CLA isomers using LA as a substrate. (C) 2013 Elsevier Ltd. All rights reserved.