WoS İndeksli Yayınlar Koleksiyonu
Permanent URI for this collectionhttps://hdl.handle.net/20.500.12573/394
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Article Citation - WoS: 16Citation - Scopus: 18Microfluidic Chip Based Direct Triple Antibody Immunoassay for Monitoring Patient Comparative Response to Leukemia Treatment(Springer, 2020-07-13) Icoz, Kutay; Akar, Unal; Unal, EkremWe report a time and cost-efficient microfluidic chip for screening the leukemia cells having three specific antigens. In this method, the target blast cells are double sorted with immunomagnetic beads and captured by the 3rd antibody immobilized on the gold surface in a microfluidic chip. The captured blast cells in the chip were imaged using a bright-field optical microscope and images were analyzed to quantify the cells. First sorting was performed with nano size immunomagnetic beads and followed by 2nd sorting where micron size immunomagnetic beads were used. The low-cost microfluidic platform is made of PMMA and glass including micro size gold pads. The developed microfluidic platform was optimized with cultured B type lymphoblast cells and tested with the samples of leukemia patients. The 8 bone marrow samples of 4 leukemia patients on the initial diagnosis and on the 15th day after the start of the chemotherapy treatment were tested both with the developed microfluidic platform and the flow cytometry. A 99% statistical agreement between the two methods shows that the microfluidic chip is able to monitor the decrease in the number of blast cells due to the chemotherapy. The experiments with the patient samples demonstrate that the developed system can perform relative measurements and have a potential to monitor the patient response to the applied therapy and to enable personalized dose adjustment.Article Citation - WoS: 3Citation - Scopus: 6High Spatial Resolution IoT Based Air PM Measurement System(Springer, 2021-04-12) Icoz, Ebru; Malik, Fasih M.; Icoz, KutayAir pollution is one of the global problems of the current era. According to World Health Organization more than 80% of the people living in metropolitan areas breathe air which exceeds the guideline limits. Particulate matter, the mixture of liquid and solid particles having diameters less than 10 mu m, is one of the important pollutants in the air. The main source of the Particulate matter is mostly burning reactions associated with industry, vehicles and homes. Several studies have shown the lethal impact of particulate matter to public health and environment. The rise of particulate matter amount in air has been linked to several health problems such as not only respiratory diseases but also mortality in infants and heart attacks. Currently, bulky stations which are high-cost and have limited spatial resolution are used to monitor the air quality. In this study we developed an alternative particulate matter measurement system which is portable and low-cost (less than 200 USD) and also integrated with cloud computing. The system allows real time distant monitoring of PM particles with high spatial resolution (meter range). The developed sensor system is able to provide air quality data in correlation with the existing stations (R-2 = 0.87). The statistical comparison between the developed system and the reference methods revealed that two systems produced statistically equal results in detecting the variations of the particulate matter.