TR-Dizin İndeksli Yayınlar Koleksiyonu
Permanent URI for this collectionhttps://hdl.handle.net/20.500.12573/396
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Article Investigation of Antiviral Potential of Food Carotenoids and Apocarotenoids Against RNA-Dependent RNA Polymerase of Hepatitis C Virus(2022-09-30) Fidan, OzkanHepatitis C disease have been a global health threat and affects a significant portion of world population. Hepatitis C have also been a silent health threat for Turkiye, where there are around half million people infected with Hepatitis C Virus (HCV). Disease burden and mortality are expected to increase gradually in the next 20 years in Turkiye. Unavailability of enough data on the currently-available drugs in routine clinical practice, their side effects and interactions with other drugs, and their efficacies on the less common genotypes indicates the necessity of alternative treatment options. Natural products from herbal and medicinal plants can indeed provide an alternative as being drug-like dietary supplements. In particular, the carotenoids and apocarotenoids are underexplored in their antiviral potential, including anti-HCV activities. Therefore, we focused on the virtual screening of various carotenoids and apocarotenoids against the RNA-dependent RNA polymerase (RdRp) of HCV. Molecular docking experiments showed strong binding affinities of the ligands to both palm and thumb domains of RdRp of HCV. In fact, some of them such as neoxanthin, crocin, canthaxanthin and cryptoflavin bound quite strongly to both domains compared to native ligands and current antiviral drugs. MD simulation for neoxanthin-RdRp complex confirmed the stability of the ligand within the binding cavity of RdRp throughout 100 ns simulation. This clearly indicated the potential of carotenoids, specifically neoxanthin, as RdRp inhibitor in treating HCV. Thus, this study not only discovered anti-HCV drug candidates with the properties of easy-to-access and low cost, but also paved the way for the development of carotenoid or apocarotenoid based dietary supplement candidates for the prevention and treatment of HCV.Article Green Synthesis of Silver Nanoparticles Using Walnut Shell Powder and Cynara sp. and Their Antibacterial Activities(2022-10-09) Erdem, İlker; Çakır, ŞerifeGümüş (Ag) ilgi çeken özellikleri ile (katalitik etkinlik, antimikrobiyal, vb.) bilinen bir malzemedir. Nano boyuttaki Gümüşartan yüzey alanı sebebiyle gelişmiş özellikler sunar. Yeşil üretim görece daha az zararlı malzemelerin kullanılması sebe - biyle umut veren daha çevre dostu bir malzeme hazırlama/üretme yöntemidir. Bu çalışmada, gümüş nano parçacıkların (Ag NP) hazırlanması için bitki özütleri (enginar, ceviz kabuğu tozu) hazırlama ortamı olarak kullanılmıştır. Farklı iki bitki özütüyle hazırlanan nano parçacıkların boyutları, sırasıyla ~46 nm ve ~109 nm olmuştur. Hazırlanan Ag NP’ların E.coli and S.aureus suşlarına karşı antibakteriyel etkileri belirlenmiş ve minimum etkili yoğunlukları araştırılmıştır.Article Tarhanadan Laktik Asit Bakterilerinin İzolasyonu(2020-07-14) Aydın, Aysun Cebeci; Çalış, Burak; Polat, Muhammet FatihTarhana is a staple food in Turkey and produced mainly by the fermentation of wheat flour and yogurt.There are variations in its use, it can be consumed as a snack, or as a soup, and its exact ingredients vary betweendifferent geographical regions. Our study aims to isolate and identify lactic acid bacteria in tarhana samples viabiochemical and molecular biology identification techniques. Three different tarhana samples were studied forbacterial Gram reactions, catalase activity, gas production, growth at 10°C and 45°C, in 6% and 16% NaClconcentrations and at pH 4,4 and pH 9,6 for the biochemical tests. For the molecular biology experiments, PCRRFLP, sequencing and RAPD-PCR were performed to identify organisms at the species and strain level.