TR-Dizin İndeksli Yayınlar Koleksiyonu

Permanent URI for this collectionhttps://hdl.handle.net/20.500.12573/396

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Publisher: search.filters.publisher.Gazi UnivSubject: search.filters.subject.Biyokimya Ve Moleküler Biyoloji

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  • Article
    Citation - WoS: 2
    Citation - Scopus: 3
    Magnetic Separation of Micro Beads and Cells on a Paper-Based Lateral Flow System
    (Gazi Univ, 2023-12-01) Farooqi, Muhammed Fuad; Icoz, Kutay
    Paper based lateral flow systems are widely used biosensor platforms to detect biomolecules in a liquid sample. Proteins, bacteria, oligonucleotides, and nanoparticles were investigated in the literature. In this work we designed a magnetic platform including dual magnets and tested the flow of micron size immunomagnetic particles alone and when loaded with cells on two different types of papers. The prewetting conditions of the paper and the applied external magnetic field are the two dominant factors affecting the particle and cell transport in paper. The images recorded with a cell phone, or with a bright field optical microscope were analyzed to measure the flow of particles and cells. The effect of prewetting conditions and magnetic force were measured, and it was shown that in the worst case, minimum 90% of the introduced cells reached to the edge of the paper. The paper based magnetophoretic lateral flow systems can be used for cell assays.
  • Article
    Citation - WoS: 1
    Citation - Scopus: 2
    Investigation of the Interaction of Adipose-Derived Mesenchymal Stem Cells With Ε-Polycaprolactone and EGG White Scaffolds
    (Gazi Univ, 2023-12-01) Oztel, Olga N.; Yilmaz, Hilal; Isoglu, I. Alper; Allahverdiyev, Adil
    The development of three-dimensional (3D) cell culture models is becoming increasingly important due to their numerous advantages over conventional monolayer culture. This study aimed to examine the interaction of adipose tissue-derived mesenchymal stem cells (AD-MSCs) with scaffolds composed of e-polycaprolactone (e-PCL) and egg white. In our study, e-PCL and egg white scaffolds were produced from their monomers by tin octoate catalyzed and heat polymerization, respectively. Characterization of e-PCL was carried out by Gel Permeation Chromatography (GPC), Fourier Transform Infrared Spectrophotometry (FTIR), Proton Nuclear Magnetic Resonance (H-NMR), Differential Scanning Calorimetry (DSC) and Scanning Electron Microscopy (SEM). AD-MSCs labeled with red fluorescent CellTracker CM-DiI were cultured on egg white and e-PCL scaffolds for 12 days. Cell viability was determined using 3-(4.5Dimethylthiazol-2yl)-2.5-diphenyltetrazolium bromide (MTT) and nitric oxide (NO) level was evaluated for toxicity. The results showed that the number of AD-MSCs in the egg white scaffold increased periodically for 12 days compared to the other groups. Although the number of ADMSCs in the e-PCL scaffold increased until day 6 of the culture, the number of cells started to decrease after day 6. These results were associated with the toxic effect of lactic acid release on cells resulting from the decomposition of e-PCL scaffolds through catabolic reactions. Therefore, these results indicated that the egg white scaffold enhanced and maintained cell adhesion and cell viability more than the e-Polycaprolactone scaffold and could be used as a scaffold in tissue engineering studies involving stem cells.