PubMed İndeksli Yayınlar Koleksiyonu

Permanent URI for this collectionhttps://hdl.handle.net/20.500.12573/397

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CRISPR/Cas9-mediated Metabolic Engineering of Endophytic Pseudomonas Loganensis Sp. Nov. for the Production of Nutritionally Valuable Carotenoids
(American Chemical Society, 2026) Arslansoy, N.; Karaman, M.Z.; Fidan, O.
Carotenoids with significant nutritional and antioxidant properties have been widely utilized in the food, feed, pharmaceutical, and cosmetic industries. They improve the nutritional value of foodstuffs and have been used as natural food colorants. However, their current supply chain is mainly dependent on extraction from plants and chemical synthesis, both of which have bottlenecks, including environmental concerns, toxicity, and allergenicity. To address global demand for sustainable and environmentally friendly production of nutrients, we engineered the endophytic Pseudomonas loganensis sp. nov. as a niche microbial chassis for nutritionally valuable carotenoid production. Using CRISPR-Cas9, we knocked out key carotenogenic genes to construct strains capable of producing zeaxanthin, lycopene, and β-carotene. Additionally, an overexpression plasmid was introduced to produce astaxanthin. HPLC analysis confirmed the successful production of four target carotenoids. The culture conditions and media compositions were optimized using response surface methodology, resulting in a ∼5-fold increase in the titers of zeaxanthin (13.4 mg/L), lycopene (9.67 mg/L), and β-carotene (23.53 mg/L), and a ∼12-fold increase in astaxanthin titer (1 mg/L) compared to LB medium without optimization. Our results indicate the potential of endophytic bacteria as a microbial chassis for carotenoid bioproduction, underscoring the potential of synthetic biology to contribute to global efforts toward nutritional security and sustainable food systems. © 2026 The Authors. Published by American Chemical Society
Citation - WoS: 1
Citation - Scopus: 1
Measuring Eudaimonic and Hedonic Wellbeing: Development and Validation of the Holistic Wellbeing Measure
(Routledge, 2025) Arslan, G.; Coşkun, M.
The primary goal of this study was to develop a concise, theoretically grounded tool –the Holistic Wellbeing Measure (HWM)– that captures both hedonic and eudaimonic facets of wellbeing. Items for the HWM were generated through a careful review of existing wellbeing scales and literature, followed by expert consultation, pilot testing, and iterative refinement to ensure conceptual coverage, clarity, and face validity. Data were collected from three distinct samples: adolescents (n = 453), young adults (n = 361), and adults (n = 358). Exploratory and confirmatory factor analyses supported a two-factor structure, with 12 items reflecting independent but related hedonic and eudaimonic wellbeing dimensions. The measure demonstrated strong internal reliability and evidence of convergent, discriminant, and concurrent validity across all age groups. Regression analyses further indicated that the HWM contributed unique variance to the prediction of general health indicators (physical, social, and mental health) and psychological problems (depression, anxiety, and somatization), above the effects of gender, age, and psychological wellbeing. These results suggest that the HWM is a valid and reliable measure for assessing both aspects of wellbeing across age groups and can support strategies aimed at promoting overall mental health. © 2025 Taylor & Francis Group, LLC.
Citation - WoS: 24
Citation - Scopus: 27
A Numerical Investigation of the GRLW Equation Using Lumped Galerkin Approach With Cubic B-Spline
(Springer International Publishing AG, 2016) Zeybek, Halil; Karakoc, S. Battal Gazi
In this work, we construct the lumped Galerkin approach based on cubic B-splines to obtain the numerical solution of the generalized regularized long wave equation. Applying the von Neumann approximation, it is shown that the linearized algorithm is unconditionally stable. The presented method is implemented to three test problems including single solitary wave, interaction of two solitary waves and development of an undular bore. To prove the performance of the numerical scheme, the error norms L-2 and L-infinity and the conservative quantities I-1, I-2 and I-3 are computed and the computational data are compared with the earlier works. In addition, the motion of solitary waves is described at different time levels.
Citation - WoS: 67
Citation - Scopus: 73
Prophylactic and Therapeutic Roles of Oleanolic Acid and Its Derivatives in Several Diseases
(Baishideng Publishing Group inc, 2020) Sen, Alaattin
Oleanolic acid (OA) and its derivatives are widely found in diverse plants and are naturally effective pentacyclic triterpenoid compounds with broad prophylactic and therapeutic roles in various diseases such as ulcerative colitis, multiple sclerosis, metabolic disorders, diabetes, hepatitis and different cancers. This review assembles and presents the latestin vivoreports on the impacts of OA and OA derivatives from various plant sources and the biological mechanisms of OA activities. Thus, this review presents sufficient data proposing that OA and its derivatives are potential alternative and complementary therapies for the treatment and management of several diseases.
Citation - WoS: 12
Role of Pretty Nanoflowers as Novel Versatile Analytical Tools for Sensing in Biomedical and Bioanalytical Applications
(Wiley, 2024) Dadi, Seyma; Ocsoy, Ismail
In recent years, an encouraging breakthrough in the synthesis of immobilized enzymes in flower-shaped called "organic-inorganic hybrid nanoflowers (hNFs)" with greatly enhanced catalytic activity and stability were reported. Although, these hNFs were discovered by accident, the enzymes exhibited highly enhanced catalytic activities and stabilities in the hNFs compared with the free and conventionally immobilized enzymes. Herein, we rationally utilized the catalytic activity of the hNFs for analytical applications. In this comprehensive review, we covered the design and use of the hNFs as novel versatile sensors for electrochemical, colorimetric/optical and immunosensors-based detection strategies in analytical perspective. Formation of nanoflowers and their biosensor function in biomedical and bioanalytical applications. image
Citation - WoS: 3
Citation - Scopus: 3
A Uniformly Valid Approximation Algorithm for Nonlinear Ordinary Singular Perturbation Problems With Boundary Layer Solutions
(Springer int Publ Ag, 2016) Cengizci, Suleyman; Atay, Mehmet Tarik; Eryilmaz, Aytekin
This paper is concerned with two-point boundary value problems for singularly perturbed nonlinear ordinary differential equations. The case when the solution only has one boundary layer is examined. An efficient method so called Successive Complementary Expansion Method (SCEM) is used to obtain uniformly valid approximations to this kind of solutions. Four test problems are considered to check the efficiency and accuracy of the proposed method. The numerical results are found in good agreement with exact and existing solutions in literature. The results confirm that SCEM has a superiority over other existing methods in terms of easy-applicability and effectiveness.

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