Biyomühendislik Ana Bilim Dalı Tez Koleksiyonu

Permanent URI for this collectionhttps://hdl.handle.net/20.500.12573/417

Browse

Browsing Biyomühendislik Ana Bilim Dalı Tez Koleksiyonu by Publisher "Abdullah Gül Üniversitesi"

Filter results by typing the first few letters
Now showing 1 - 3 of 3
  • Thumbnail Image
    Master Thesis
    Lösemi Hücrelerinin Hücre Yüzey Ayıraçları ile İmmünomanyetik Ayrıştırılması ve Sabitlenmesi
    (Abdullah Gül Üniversitesi, 2017) GERÇEK, TAYYİBE; Gerçek, Tayyibe; İçöz, Kutay
    Akut Limfoblastik Lösemi, kısaca ALL, özellikle B öncüllü Akut Limfoblastik Lösemi çocukluk kanserleri arasında en yaygın olan kan malignitesidir. Löseminin farklı çeşitlerde tedavileri bulunmaktadır ancak terapiden sonra hastanın vücudunda kalan kanser hücrelerinin yüzünden yıllar içinde hastalığın tekrarlama ihtimali vardır. Fakat terapiden sonra kalan bu kanser hücreleri rutin klinik takip testlerinde görünmemektedir. Bu tarz lösemi gibi hastalıklar Minimal Kalıntı Hastalığı (Minimal Residual Disease-MRD) olarak adlandırılır. Günümüzde MRD tayini için yalnızca iki yol bulunmaktadır. Bunlar akım sitometrisi ve eş zamanlı polimeraz zincir reaksiyonudur. Birçok farklı laboratuvarda bu cihazlardan bulunmasına rağmen, cihazlar MRD tayini için kalibre olmak zorundadır. Bugünlerde MRD tayininin gerekli olduğu konusunda bir görüş birliği vardır ancak nasıl ve ne zaman yapılması gerektiği konusu yetkililer tarafından hala tartışılmaktadır. Bu projenin nihai hedefi MRD tayin edebilen bir çip üretmektir. Bu çalışmayla ise nano ve mikro boyutlarda manyetik boncuklar kullanarak lösemi hücrelerini yakalamaya çalışıyoruz. Bu manyetik boncuklar, lösemi hücrelerinin membranında bulunan CD19 ve CD45 işaretleyicileriyle kaplanmıştır. Manyetik boncuklarla hücreleri yakaladıktan sonraki adım onları yüzeye sabitlemektir. Altın yüzeyler kullanılmakta ve gerekli antikorlarla işlevsel hale getirilmektedir. Böylelikle bir immunosandviç yapısı oluşmakta ve hücreler yüzeye sabitlenmektedir.
  • Thumbnail Image
    Master Thesis
    Centella asiatica extract containing bilayered electrospun wound dressing
    (Abdullah Gül Üniversitesi, 2019) KOÇ, NURAY
    Innovative and bioactive wound dressings prepared by electrospinning mimicking the native structure of the extracellular matrix (ECM) have gained significant interest as an alternative to conventional wound care applications. In this study, bilayered wound dressing material was produced by sequential electrospinning of quaternized poly(4- vinyl pyridine) (upper layer) on the Centella Asiatica (CA) extract containing electrospun poly(D, L-lactide-co-glycolide) (PLGA)/poly(3-hydroxybutyrate-co-3- hydroxy valerate) (PHBV) blend membrane (lower layer). Scanning electron microscopy (SEM) was utilized to show a uniform and bead-free fiber structure of electrospun membranes. The average diameter of CA extract containing electrospun PLGA/PHBV blend membrane was calculated 0.471±0.11 µm, whereas the average fiber diameter of electrospun poly(Q-VP) membranes was in the range of 0.460±0.057 µm. Chemical, thermal, mechanical properties, and adsorption capacity of electrospun membranes, as well as the cumulative release of CA from the electrospun PLGA/PHBV membrane, were investigated. Viability, adhesion, and attachment of human fibroblast cells on the electrospun membranes on pre-set days were evaluated by the colorimetric CellTiter 96® Aqueous One Solution Cell Proliferation Assay (MTS assay) and SEM. Results revealed that CA loaded bilayered electrospun wound dressing showed promoted attachment and proliferation of fibroblasts. Hence, it can be concluded that CA extract containing bilayered electrospun wound dressing prepared in this study has a promising potential for wound healing applications.
  • Thumbnail Image
    Master Thesis
    Kolanjiyokarsinoma Proliferasyonunun Otofaji ve Hedgehog Sinyal Yolaklarının İnhibisyonu ile Azaltılması
    (Abdullah Gül Üniversitesi, 2019) AKTAŞ, NİHAN; Aktaş, Nihan; Khatıb, Mona El
    Cholangiocarcinoma (CCA) is the second most common liver cancer type. The median survival rate of CCA patients is really low. Aberrant signaling pathways such as PI3K/AKT/mTOR pathway could be main drivers in CCA pathogenesis. Hedgehog (Hh) pathway is also dysregulated in several carcinomas including CCA. It regulates and crosstalks with autophagy, which is a lysosomal degradation process. There is no study showing the crosstalk between Hh pathway and autophagy in the context of CCA. Since both autophagy and Hh pathways are dysregulated in CCA, better understanding of how they crosstalk with each other and contribute to CCA pathogenesis is important. Considering this crosstalk between Hh pathway and autophagy, we conducted a combination treatment comprising Hh and autophagy pathway inhibitors in EGI-1 and TFK-1 CCA cell lines. In our study, we firstly checked anti-proliferative effects of Hh pathway inhibitor, GANT61, and different autophagy blockers using MTT and Annexin V assay and cell cycle analysis. After determination of IC30 of GANT61 (15 uM), chloroquine (25 uM for TFK-1 and 50 uM for EGI-1), and nocodazole (0.2 uM for EGI-1 and 0.4 uM for TFK-1), we conducted combination experiments. When we inhibit Hh pathway with targeting different steps of autophagy, we observed that proliferation of both EGI-1 and TFK-1 cells decreased compared to single treatments. After that, we checked the expression of autophagy-related LC3B protein and Akt, a negative regulator of autophagy, using western blotting after single treatments and combinational treatments. Based on the change in LC3B and Akt expression, we also concluded that, inhibition of autophagy with Hh pathway either induce or inhibit autophagy depends on the administered treatments. This study highlights the importance of deciphering the exact mechanisms that control autophagy in CCA, thus leading to better treatment.