Biyomühendislik Ana Bilim Dalı Tez Koleksiyonu

Permanent URI for this collectionhttps://hdl.handle.net/20.500.12573/417

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Centella asiatica extract containing bilayered electrospun wound dressing
(Abdullah Gül Üniversitesi, 2019) KOÇ, NURAY; AGÜ, Yaşam ve Doğa Bilimleri Fakültesi, Biyomühendislik Bölümü; KOÇ, NURAY
Innovative and bioactive wound dressings prepared by electrospinning mimicking the native structure of the extracellular matrix (ECM) have gained significant interest as an alternative to conventional wound care applications. In this study, bilayered wound dressing material was produced by sequential electrospinning of quaternized poly(4- vinyl pyridine) (upper layer) on the Centella Asiatica (CA) extract containing electrospun poly(D, L-lactide-co-glycolide) (PLGA)/poly(3-hydroxybutyrate-co-3- hydroxy valerate) (PHBV) blend membrane (lower layer). Scanning electron microscopy (SEM) was utilized to show a uniform and bead-free fiber structure of electrospun membranes. The average diameter of CA extract containing electrospun PLGA/PHBV blend membrane was calculated 0.471±0.11 µm, whereas the average fiber diameter of electrospun poly(Q-VP) membranes was in the range of 0.460±0.057 µm. Chemical, thermal, mechanical properties, and adsorption capacity of electrospun membranes, as well as the cumulative release of CA from the electrospun PLGA/PHBV membrane, were investigated. Viability, adhesion, and attachment of human fibroblast cells on the electrospun membranes on pre-set days were evaluated by the colorimetric CellTiter 96® Aqueous One Solution Cell Proliferation Assay (MTS assay) and SEM. Results revealed that CA loaded bilayered electrospun wound dressing showed promoted attachment and proliferation of fibroblasts. Hence, it can be concluded that CA extract containing bilayered electrospun wound dressing prepared in this study has a promising potential for wound healing applications.
The effect of hedgehog inhibition and autophagy modulation on the proliferation and survival of AML cell lines /
(2019) ŞANSAÇAR, MERVE; AGÜ, Yaşam ve Doğa Bilimleri Fakültesi, Biyomühendislik Bölümü; ŞANSAÇAR, MERVE
Acute myeloid leukemia (AML) is a heterogeneous hematopoietic malignancy which occurs as a result of many chromosomal abnormalities such as translocations, deletions or insertions. Aberrant signaling pathways such as PI3K/AKT/mTOR, Notch and Hedgehog pathway have a role in the pathogenesis of AML. Hedgehog pathway (Hh) is a conserved signalling pathway that is important during embryogenesis. It crosstalks with other pathways and regulate autophagy, a cellular degradation and organelle turnover process. Several studies suggested that autophagy modulation could act as an escape mechanism in AML. Given the role of autophagy and Hh in AML, understanding the relationship between autophagy and Hh pathway is important to overcome the leukemic growth. Hence, we checked the effect of Hh inhibition using GANT61 on MOLM-13 and CMK cells using MTT cell viability assay. GANT61 led to a decrease in the both MOLM-13 and CMK cells. After that, we sought to understand the effect of autophagy modulation on CMK and MOLM-13 cell lines and we have found that autophagy inhibitors, NH4CI, Chloroquine(CQ), Hydroxychloroquine and Nocodazole lead to a decrease in the proliferation of CMK and MOLM-13 cell lines. However, PP242, an autophagy activator, had no effect on the proliferation of CMK and MOLM-13 cell lines. Combination treatment of autophagy modulators and GANT61 had a synergistic effect on MOLM-13 but not on CMK. GANT61 treatment increased autophagy in AML cell lines that were correlated with an increase in the expression of LC3B-II detected by western blotting. Also, combination treatment with nocodazole and GANT61 elevated that increase in LC3B-II in both MOLM-13 and CMK cell lines. AKT protein expression changed depending on the type of treatment and cell lines. In conclusion, targeting of Hh and autophagy is a promising therapy against MOLM-13 cell line but not against CMK.
Immunomagnetic separation and immobilization of leukemia cells using cell surface markers /
(Abdullah Gül Üniversitesi, 2017) GERÇEK, TAYYİBE; AGÜ, Yaşam ve Doğa Bilimleri Fakültesi, Biyomühendislik Bölümü; GERÇEK, TAYYİBE
Acute Lympoblastic Leukemia, shortly ALL, especially B-precursor Acute Lymphoblastic Leukemia is the most common blood malignancies among childhood cancers. There are different kinds of leukemia treatments but after those therapies, there is likelihood of relapsing the cancer in years because of remained cancer cells in the patient’s body. Nevertheless remained cancer cells in the body after therapy cannot be seen in the routine clinical follow-up tests. Those kind of diseases like leukemia are named as Minimal Residual Disease (MRD). Currently, there are only two ways to detect Minimal Residual Disease. These are flow cytometry and real-time quantified polymerase chain reaction (RQ-PCR). However many different labs have these Flow Cytometry and RQ-PCR, both devices should be calibrated to detect MRD cells. Nowadays there is a consensus about requiring MRD detection but still it is discussed by the authorities about when and how it should be done. The ultimate aim of this project is to produce a chip that can detect MRD cells. With these work we try to capture leukemic cells by using magnetic beads in nano and microsize. These magnetic beads are coated with CD19 and CD45 antibody markers that are in the membrane of leukemia cells. Next step after capturing them with magnetic beads is to immobilize them onto a surface. Golden surfaces are used and functionalized by antibodies. Thus an immunosandwich structure is occured and the cells are immobilized.

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