WoS İndeksli Yayınlar Koleksiyonu
Permanent URI for this collectionhttps://hdl.handle.net/20.500.12573/394
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Article Citation - WoS: 14Citation - Scopus: 15Image-Analysis Based Readout Method for Biochip: Automated Quantification of Immunomagnetic Beads, Micropads and Patient Leukemia Cell(Pergamon-Elsevier Science Ltd, 2020-06) Uslu, Fatma; Icoz, Kutay; Tasdemir, Kasim; Dogan, Refika S.; Yilmaz, BulentFor diagnosing and monitoring the progress of cancer, detection and quantification of tumor cells is utmost important. Beside standard bench top instruments, several biochip-based methods have been developed for this purpose. Our biochip design incorporates micron size immunomagnetic beads together with micropad arrays, thus requires automated detection and quantification of not only cells but also the micropads and the immunomagnetic beads. The main purpose of the biochip is to capture target cells having different antigens simultaneously. In this proposed study, a digital image processing-based method to quantify the leukemia cells, immunomagnetic beads and micropads was developed as a readout method for the biochip. Color, size-based object detection and object segmentation methods were implemented to detect structures in the images acquired from the biochip by a bright field optical microscope. It has been shown that manual counting and flow cytometry results are in good agreement with the developed automated counting. Average precision is 85 % and average error rate is 13 % for all images of patient samples, average precision is 99 % and average error rate is 1% for cell culture images. With the optimized micropad size, proposed method can reach up to 95 % precision rate for patient samples with an execution time of 90 s per image.Article Citation - WoS: 4Citation - Scopus: 4Camera-Based Wildfire Smoke Detection for Foggy Environments(SPIE - Society of Photo-Optical Instrumentation Engineers, 2022-10-27) Tas, Merve; Tas, Yusuf; Balki, Oguzhan; Aydin, Zafer; Tasdemir, Kasim; Aydln, ZaferSmoke is the first visible sign of forest fires and the most commonly used feature for early forest fire detection using data from cameras. However, one of the natural challenges is the dense fog that appears in forests, which decreases the detection accuracy or triggers false alarms. In this study, we propose a system with a deep neural network-based image preprocessing approach that significantly improves the smoke segmentation and classification performance by dehazing the camera view. Our experimental results provide that the classification models reach 99% F1 score for the correct classification of smoke when the image dehazing method is used before the training process. The smoke localization system achieves 60% average precision when the mask region-based convolutional neural network is used with the ResNet101-FPN backbone. The proposed approach can be utilized for all smoke segmentation frameworks to increase fire detection performance. (c) 2022 SPIE and IS&T