WoS İndeksli Yayınlar Koleksiyonu
Permanent URI for this collectionhttps://hdl.handle.net/20.500.12573/394
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Article Engineering a Bilayered Scaffold as a Potential Cardiac Patch: From Scaffold Design to in Vitro Assessment(Springer Singapore Pte Ltd, 2025-11-24) Yuruk, Adile; Duzler, Ayhan; Isoglu, Sevil Dincer; Isoglu, Ismail AlperIn this study, we developed a novel bilayered scaffold consisting of a bottom layer composed of the Decellularized Bovine Pericardium (DP) coated with Polyaniline Nanoparticles (PANINPs) and a top layer made of an electrospun Poly(lactic-co-glycolic acid)/Gelatin (PLGA/Gel) membrane incorporated with Vascular Endothelial Growth Factor (VEGF) and hawthorn extract. Functionally, the DP supplies native Extracellular Matrix (ECM) components and mechanical support, while PANINPs provide conductivity. The electrospun PLGA/Gel layer mimics fibrous ECM. It incorporates bioactives, with VEGF promoting pro-angiogenic stimulation and hawthorn extract enhancing anticoagulant activity, as well as increasing surface hydrophilicity. The tissue adhesive ensures the interfacial integrity between the two layers. Decellularization efficiency was confirmed histologically using 4 ',6-diamidino-2-phenylindole (DAPI) and Hematoxylin-Eosin (H&E) staining. The DP exhibited a DNA content of 115.9 +/- 47.8 ng/mg DNA, compared to 982.88 +/- 395.42 ng/mg in Native Pericardium (NP). The PANINPs had an average particle size of 104.94 +/- 13.7 nm. The conductivity of PANINPs-coated decellularized pericardium was measured to be 9.093 +/- 8.6 x 10- 4 S/cm using the four-point probe method. PLGA/Gel membranes containing hawthorn extract (1%, 5%, 10%, and 15% w/v) and VEGF (0.1 mu g/mL, 0.5 mu g/mL, and 1 mu g/mL) were fabricated by electrospinning, resulting in fiber diameters between 850 and 1200 nm and pore sizes between 14 and 20 mu m. The anticoagulant efficiency of the membranes containing hawthorn extract reached 430 s in the Activated Partial Thromboplastin Time Assay (aPTT). Mechanical testing revealed a tensile strength of 22.70 +/- 6.33 MPa, an elongation of 53.58 +/- 10.63%, and Young's modulus of 0.67 +/- 0.10 MPa. The scaffold also exhibited over 91% cell viability and excellent cardiomyocyte adhesion. The hemolysis ratio was determined to be 0.421 +/- 0.191%, which confirms its blood compatibility. Our results indicate that the proposed bilayered scaffold can be a promising candidate for cardiac patch applications.Conference Object Peptide Targeted Core Cross-Linked Micelles for Dox Delivery to HER2 Expressing Cancer Cells(Mary Ann Liebert, inc, 2022) Bayram, Nazende Nur; Ulu, Gizem Tugce; Gurdap, Seda; Isoglu, Ismail Alper; Baran, Yusuf; Isoglu, Sevil DincerArticle Citation - WoS: 1Citation - Scopus: 1Enhancing Bioink Potential of Hyaluronic Acid by Microwave-Induced Methacrylation(Elsevier, 2025-10) Ishtyah, Yazan R. B.; Cosgun, Seyma Nur Kirmic; Ceylan, Deniz; Demirtas, Tugrul Tolga; Isoglu, Sevil DincerThis study reports the development of a light-curable methacrylated hyaluronic acid (HAMA) synthesized using microwave irradiation. The methacrylation process was carried out with AEMA as the methacrylating agent via an EDC/NHS protocol at varying microwave energy levels and compared comprehensively with those synthesized using the conventional heating method. The HAMA synthesis by microwave was optimized by applying different power levels (100 W, 250 W, and 800 W). The products were characterized by 1H NMR to determine the degree of methacrylation (DoM). The microwave-assisted synthesis significantly reduced the reaction time from 24 h to 6 min, improved reaction efficiency, and shortened the purification period from 3 days to 1 day. Additionally, it enhanced the mechanical, rheological, and swelling properties of the resulting hydrogels. The highest DoM was achieved at 78 % for HAMA-100 hydrogels synthesized at 100 W microwave energy. Rheological analysis demonstrated that microwave-assisted HAMA hydrogels could withstand nearly 100 % strain, outperforming those produced by conventional methods. This indicated the presence of an improved energy distribution mechanism at the molecular level within the polymer network structure of the microwave-assisted hydrogels. It was also observed that the microwave-assisted hydrogels exhibited strain-hardening behavior, ensuring the stability of bioactive structures in bioinks. Furthermore, the printing conditions for HAMA-100 gels were optimized in terms of printing pressure and speed. These findings highlight the significant role of microwave energy in achieving superior hydrogel properties, making it a promising green method for preparing bioinks for 3D printing applications.Article Citation - WoS: 36Citation - Scopus: 40Advances in Micelle-Based Drug Delivery: Cross-Linked Systems(Bentham Science Publ Ltd, 2017-04-04) Isoglu, Ismail Alper; Ozsoy, Yildiz; Isoglu, Sevil DincerThere are several barriers that drug molecules encounter in body beginning from kidney filtration and reticulo-endothelial system (RES) clearance to cellular trafficking. Multifunctional nanocarriers have a great potential for the delivery of drugs by enhancing therapeutic activity of existing methodologies. A variety of nanocarriers are constructed by different material types, which have unique physicochemical properties for drug delivery applications. Micelles formed by amphiphilic polymers are one of the most important drug/nanocarrier formulation products, in which the core part is suitable for encapsulation of hydrophobic agent whereas the outer shell can be utilized for targeting the drug to the disease area. Micelles as self-assembled nanostructures may encounter difficulties in biodistribution of encapsulated drugs because they have a tendency to be dissociated in dilution or high ionic strength. Therefore, therapeutic efficiency is decreased and it requires high amount of drug to be administered to achieve more efficient result. To overcome this problem, covalently stabilized structures produced by cross-linking in core or shell part, which can prevent the micelle dissociation and regulate drug release, have been proposed. These systems can be designed as responsive systems in which cross-links are degradable or hydrolysable under specific conditions such as low pH or reductive environment. These are enhancing characteristics in drug delivery because their cleavage allows the release of bioactive agent encapsulated in the carrier at a certain site or time. This review describes the chemical methodologies for the preparation of cross-linked micelles, and reports an update of latest studies in literature.