PubMed İndeksli Yayınlar Koleksiyonu
Permanent URI for this collectionhttps://hdl.handle.net/20.500.12573/397
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Article Citation - WoS: 11Citation - Scopus: 13The Effect of Seed Sludge Type on Aerobic Granulation via Anoxic-Aerobic Operation(Taylor & Francis Ltd, 2014-06-17) Ersan, Yusuf Cagatay; Erguder, Tuba HandeThe effects of two seed sludge types, namely conventional activated sludge (CAS) and membrane bioreactor sludge (MBS), on aerobic granulation were investigated. The treatment performances of the reactors were monitored during and after the granulation. Operational period of 37 days was described in three phases; Phase 1 corresponds to Days 1-10, Phase 2 (overloading conditions) to Days 11-27 and Phase 3 (recovery) to Days 28-37. Aerobic granules of 0.56 +/- 0.23 to 2.48 +/- 1.28mm were successfully developed from both MBS and CAS. First granules appeared on Day 9 in both reactors, indicating that there was no difference between two seed sludge types in terms of the time period for granulation initiation. The results revealed that the granules developed from MBS performed better than CAS in terms of settleability, stability, biomass retention, adaptation, protection of granular structure at high loading rates (0.86 gN/L d and 3.92 gCOD/Ld) and low COD/TAN ratio (5). Granules of MBS were also found to be capable of providing better protection for nitrifiers at toxic free-ammonia concentrations (38-46 mg/L NH3-N), thus showing better treatment recovery than those of CAS.Article Citation - WoS: 4Citation - Scopus: 6One-Pot Conjugated Linoleic Acid Production From Castor Oil by Rhizopus Oryzae Lipase and Resting Cells of Lactobacillus Plantarum(Taylor & Francis Ltd, 2017-10-03) Khaskheli, Abid Ali; Talpur, Farah Naz; Aydin, Aysun Cebeci; Jawaid, Sana; Surhio, Muhammad Ali; Afridi, Hassan ImranConjugated linoleic acid (CLA) has attracted as novel type of fatty acids having unusual health-promoting properties such as anticarcinogenic and antiobesitic effects. The present work employed castor oil as substrate for one-pot production of CLA using washed cells of Lactobacillus plantarum (L. plantarum) and lipases as catalysts. Among the screened lipases, the lipase Rhizopus oryzae (ROL) greatly assisted resting cells to produce CLA. Mass spectral analysis of the product showed that two major isomers of CLA were produced in the reaction mixture i.e. cis-9, trans-11 56.55% and trans-10, cis-12 43.45%. Optimum factors for CLA synthesis were found as substrate concentration (8mg/mL), pH (6.5), washed cell concentration (12% w/v), and incubation time of 20h. Hence, the combination of ROL with L. plantarum offers one pot production of CLA selectively using castor oil as a cost-effective substrate.Article Citation - WoS: 1Citation - Scopus: 1Concurrent Inhibition of FLT3 and Sphingosine Kinase-1 Triggers Synergistic Cytotoxicity in Midostaurin Resistant FLT3-ITD Positive Acute Myeloid Leukemia Cells via Blocking FLT3/TAT5A Signaling to Induce Apoptosis(Taylor & Francis Ltd, 2025-03-21) Tecik, Melisa; Adan, AysunThe FMS-like tyrosine kinase 3-internal tandem duplication (FLT3-ITD) is one of the most frequent mutations observed in acute myeloid leukemia (AML) which contributes to disease progression and unfavorable prognosis. Midostaurin, a small FLT3 inhibitor (FLT3I), is clinically approved. However, patients generally possess acquired resistance when midostaurin used alone. Shifting the balance in the sphingolipid rheostat toward anti-apoptotic sphingosine kinase-1 (SK-1) or glucosylceramide synthase (GCS) is related to therapy resistance in cancer, however, their role in midostaurin resistant FLT3-ITD positive AML has not been previously investigated. We generated midostaurin resistant MV4-11 and MOLM-13 cell lines which showed increased IC50 values compared to their sensitive partner cells. SK-1 is overexpressed in resistant cells while GCS remains unchanged. Subsequent pharmacological targeting of SK-1 in resistant cells decreased SK-1 protein level, inhibited cell proliferation and showed additive or synergistic effect on cell growth, as confirmed by the Chou-Talalay combination index, and induced G0/G1 arrest (PI staining by flow cytometry). Cotreatment (SKI-II plus midostaurin) triggered apoptosis via phosphatidylserine exposure (annexin V/PI double staining). Mechanistically, induction of the intrinsic pathway of apoptosis was confirmed as increased activating cleavages of caspase-3 and PARP and increased Bax/Bcl-2 ratios. Activating phosphorylations of FLT3 (at tyrosine residue 591) and STAT5A (at tyrosine residue 694) dramatically inhibited in resistant cells treated with the combination. In conclusion, midostaurin resistance could be reversed by dual SK-1 and FLT3 inhibition in midostaurin resistant AML cell lines, providing the first evidence of a novel treatment approach to re-sensitize FLT3-ITD positive AML.