PubMed İndeksli Yayınlar Koleksiyonu
Permanent URI for this collectionhttps://hdl.handle.net/20.500.12573/397
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Article CRISPR/Cas9-mediated Metabolic Engineering of Endophytic Pseudomonas Loganensis Sp. Nov. for the Production of Nutritionally Valuable Carotenoids(American Chemical Society, 2026-01-02) Arslansoy, N.; Karaman, M.Z.; Fidan, O.Carotenoids with significant nutritional and antioxidant properties have been widely utilized in the food, feed, pharmaceutical, and cosmetic industries. They improve the nutritional value of foodstuffs and have been used as natural food colorants. However, their current supply chain is mainly dependent on extraction from plants and chemical synthesis, both of which have bottlenecks, including environmental concerns, toxicity, and allergenicity. To address global demand for sustainable and environmentally friendly production of nutrients, we engineered the endophytic Pseudomonas loganensis sp. nov. as a niche microbial chassis for nutritionally valuable carotenoid production. Using CRISPR-Cas9, we knocked out key carotenogenic genes to construct strains capable of producing zeaxanthin, lycopene, and β-carotene. Additionally, an overexpression plasmid was introduced to produce astaxanthin. HPLC analysis confirmed the successful production of four target carotenoids. The culture conditions and media compositions were optimized using response surface methodology, resulting in a ∼5-fold increase in the titers of zeaxanthin (13.4 mg/L), lycopene (9.67 mg/L), and β-carotene (23.53 mg/L), and a ∼12-fold increase in astaxanthin titer (1 mg/L) compared to LB medium without optimization. Our results indicate the potential of endophytic bacteria as a microbial chassis for carotenoid bioproduction, underscoring the potential of synthetic biology to contribute to global efforts toward nutritional security and sustainable food systems. © 2026 The Authors. Published by American Chemical SocietyArticle Citation - WoS: 2Citation - Scopus: 2Measuring Eudaimonic and Hedonic Wellbeing: Development and Validation of the Holistic Wellbeing Measure(Routledge, 2025-10-09) Arslan, G.; Coşkun, M.The primary goal of this study was to develop a concise, theoretically grounded tool –the Holistic Wellbeing Measure (HWM)– that captures both hedonic and eudaimonic facets of wellbeing. Items for the HWM were generated through a careful review of existing wellbeing scales and literature, followed by expert consultation, pilot testing, and iterative refinement to ensure conceptual coverage, clarity, and face validity. Data were collected from three distinct samples: adolescents (n = 453), young adults (n = 361), and adults (n = 358). Exploratory and confirmatory factor analyses supported a two-factor structure, with 12 items reflecting independent but related hedonic and eudaimonic wellbeing dimensions. The measure demonstrated strong internal reliability and evidence of convergent, discriminant, and concurrent validity across all age groups. Regression analyses further indicated that the HWM contributed unique variance to the prediction of general health indicators (physical, social, and mental health) and psychological problems (depression, anxiety, and somatization), above the effects of gender, age, and psychological wellbeing. These results suggest that the HWM is a valid and reliable measure for assessing both aspects of wellbeing across age groups and can support strategies aimed at promoting overall mental health. © 2025 Taylor & Francis Group, LLC.Book Part Citation - Scopus: 1Measurement of Autophagic Activity in Cancer Cells With Flow Cytometric Analysis Using Cyto-Id Staining(Humana Press Inc., 2024) Şansaçar, Merve; Gencer Akçok, Emel BaşakAutophagy is an evolutionarily conserved process providing the energy that cells need to survive, especially in stress situations, through catabolic processes. Considering the dual role of autophagy in cancer cells depending on the cellular context, it is crucial to comprehend the effect of drug candidates put forward to prevent cancer through the autophagy pathway. The CYTO-ID® Autophagy Detection Kit allows a rapid, specific and quantitative measurement of autophagic activity at the cellular level using a 488 nm-excitable green fluorescent detection reagent via flow cytometer. In this chapter, we present the CYTO-ID® Autophagy Detection method with a stepwise protocol to monitor the autophagy flux after the application of any compound to suspension cancer cell lines with flow cytometric analysis. © 2025 Elsevier B.V., All rights reserved.Article Evaluation of HOTAIR, HOXD8, HOXD9, HOXD11 Gene Expression Levels in Turkish Patients With Acute and Chronic Myeloid Leukemia: A Single Center Experience(Cellular and Molecular Biology Association, 2024-11-27) Saraymen, Esma; Erdem, Yakut; Akalin, Hilal Ünlü; Taşçıoğlu, Nazife; Saraymen, Berkay; Celik, Serhat; Özkul, Yusuf T.Homeobox (HOX) transcript antisense RNA (HOTAIR) and HOX genes are reported to be more expressed in various cancers in humans in recent studies. The role of HOTAIR and HOXD genes in acute myeloid leukemia (AML) and chronic myeloid leukemia (CML) is not well known. In this study, expression levels of HOXD8, HOXD9 and HOXD11 from HOXD gene family and HOTAIR were determined from peripheral blood samples of 30 AML and 30 CML patients and 20 healthy volunteers by quantitative Real Time PCR. We determined that the expression levels of HOXD9 and HOXD11 in the AML patients were significantly lower than the control group (p<0.001 and p=0.002, respectively). There was no significant difference in the expression levels of HOTAIR and HOXD8 when compared to the control group. In the CML patients there was a significant increase in the expression level of HOTAIR when compared to the control group (p=0.002). The expression levels of HOXD9 and HOXD11 were found to be significantly lower than the control group (p<0.001). Our study showed that HOTAIR may not be a biomarker in the diagnosis and is not significantly correlated with the clinicopathological prognostic characteristics of AML. Additionally; it can be said that HOTAIR is oncogenic by suppressing the expression of HOXD9 and HOXD11 but not HOXD8 in CML patients. The expression profiles of HOTAIR may be a potential biomarker in the diagnosis of CML patients in predicting and monitoring drug resistance. © 2025 Elsevier B.V., All rights reserved.Article Citation - WoS: 16Citation - Scopus: 20Convenient Site Selection of a Floating PV Power Plant in Türkiye by Using GIS-Fuzzy Analytical Hierarchy Process(Springer Heidelberg, 2024-02-28) Karipoglu, Fatih; Koca, Kemal; Ilbahar, EsraFloating photovoltaics (FPVs) are appearing as a promising and an alternative renewable energy opinion in which PV panels are mounted on floating platforms in order to produce electricity from renewable energy on water such as seas, dams, rivers, oceans, canals, fish farms, and reservoirs. So far, such studies related to the body knowledge on financial, technical, and environmental aspects of installation of FPV have not been performed in Turkey while expanding steadily in other countries. In this study, suitable site selection for installation of FPV power plants on three lakes in Turkey was studied by performing geographic information system (GIS) and the fuzzy analytic hierarchy process (FAHP) as multi-criteria decision-making (MCDM) method. This detailed study revealed that the criterion of global horizontal irradiance (GHI) was determined as the most crucial criterion for the installation of FPV on Beysehir Lake, Lake of Tuz, and Van Lake. Additionally, it was clearly seen that the Beysehir Lake had the highest value approximately 52% among other lakes for installation, that is why Beysehir Lake is selected as the best option for installation of an FPV system with this multi-criteria approach.Article Citation - Scopus: 24Analyzing the Nexus Between Environmental Sustainability and Clean Energy for the USA(Springer, 2024-03-22) Dogan, Eyup; Si Mohammed, Kamel; Khan, Zeeshan Anis; BinSaeed, Rima Hassan; Mohammed, Kamel SiEnvironmental sustainability is a key target to achieve sustainable development goals (SDGs). However, achieving these targets needs tools to pave the way for achieving SDGs and COP28 targets. Therefore, the primary objective of the present study is to examine the significance of clean energy, research and development spending, technological innovation, income, and human capital in achieving environmental sustainability in the USA from 1990 to 2022. The study employed time series econometric methods to estimate the empirical results. The study confirmed the long-run cointegrating relationship among CO<inf>2</inf> emissions, human capital, income, R&D, technological innovation, and clean energy. The results are statistically significant in the short run except for R&D expenditures. In the long run, the study found that income and human capital contribute to further aggravating the environment via increasing CO<inf>2</inf> emissions. However, R&D expenditures, technological innovation, and clean energy help to promote environmental sustainability by limiting carbon emissions. The study recommends investment in technological innovation, clean energy, and increasing R&D expenditures to achieve environmental sustainability in the USA. © 2024 Elsevier B.V., All rights reserved.