Browsing by Author "Khaskheli, Abid Ali"

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Citation - WoS: 47
Citation - Scopus: 49
Monitoring the Rhizopus Oryzae Lipase Catalyzed Hydrolysis of Castor Oil by ATR-FTIR Spectroscopy
(Elsevier, 2015) Khaskheli, Abid Ali; Talpur, Farah N.; Ashraf, Muhammad Aqeel; Cebeci, Aysun; Jawaid, Sana; Afridi, Hassan Imran
A rapid and environmental friendly Attenuated total reflectance Fourier-transform infrared (ATR-FTIR) spectroscopic method was developed for monitoring the Rhizopus oryzae lipase (ROL) catalyzed hydrolysis of castor oil in oil-in-water emulsion system. A calibration curve was constructed using partial least square (PLS) model by gravimetric addition of oleic acid (10-50%) in castor oil to detect the carbonyl absorption of free fatty acids (FFA) in the region (1690-1730 cm(-1)). The correlation co-efficient (R-2) and root mean square error of calibration (RMSEC) by PIS model were found to be 0.999 and 0.316, respectively. ROL was found to be an efficient biocatalyst to produce free fatty acids (FFA) from castor oil. Factors affecting the rate of hydrolysis such as enzyme concentration (0.01%, w/v), pH (7), temperature (37 degrees C), oil-water ratio (1:4) and reaction time (12 h) were optimized. Under all set of conditions the ROL effectively hydrolyzed castor oil up to 90% yield of fatty acids. The methodology is fairly environmental friendly in both cases, i.e. using lipase for hydrolysis of castor oil and analyzing the product through FTIR spectroscopy. (C) 2015 Elsevier B.V. All rights reserved.
Citation - WoS: 4
Citation - Scopus: 6
One-Pot Conjugated Linoleic Acid Production From Castor Oil by Rhizopus Oryzae Lipase and Resting Cells of Lactobacillus Plantarum
(Taylor & Francis Ltd, 2017) Khaskheli, Abid Ali; Talpur, Farah Naz; Aydin, Aysun Cebeci; Jawaid, Sana; Surhio, Muhammad Ali; Afridi, Hassan Imran
Conjugated linoleic acid (CLA) has attracted as novel type of fatty acids having unusual health-promoting properties such as anticarcinogenic and antiobesitic effects. The present work employed castor oil as substrate for one-pot production of CLA using washed cells of Lactobacillus plantarum (L. plantarum) and lipases as catalysts. Among the screened lipases, the lipase Rhizopus oryzae (ROL) greatly assisted resting cells to produce CLA. Mass spectral analysis of the product showed that two major isomers of CLA were produced in the reaction mixture i.e. cis-9, trans-11 56.55% and trans-10, cis-12 43.45%. Optimum factors for CLA synthesis were found as substrate concentration (8mg/mL), pH (6.5), washed cell concentration (12% w/v), and incubation time of 20h. Hence, the combination of ROL with L. plantarum offers one pot production of CLA selectively using castor oil as a cost-effective substrate.
Citation - WoS: 9
Citation - Scopus: 13
A Highly Selective Whole Cell Biocatalysis Method for the Production of Two Major Bioactive Conjugated Linoleic Acid Isomers
(Elsevier, 2013) Khaskheli, Abid Ali; Talpur, Farah N.; Demir, Ayhan S.; Cebeci, Aysun; Jawaid, Sana
Conjugated linoleic acid (CLA) is well known potential anticarcinogenic, antiatherogenic and a number of other therapeutic qualities bearing substance. In the present study we have isolated Lactobacillus plantarum from local dairy buffalo milk and used as a whole cell biocatalyst, for selective CLA isomer production from linoleic acid (LA). Quantitative analysis revealed that the cis-9, trans-11 CLA comprised of 51% (w/w), while trans-10, cis-12 CLA accounted for 49% (w/w) of total CLA produced by L. plantarum. The product formation and quantitation was monitored by the spectrophotometric method and the individual isomers separation was done with GCFID. After optimization of biotransformation conditions such as pH, LA concentration, effect of preincubation, fermentation time; the highest CLA production (19.07% conversion) was achieved at 120 h in the presence of 1.62 mg ml-1 of LA. The results revealed that the bacterium is an efficient biocatalyst for the production of two major bioactive CLA isomers using LA as a substrate. (C) 2013 Elsevier Ltd. All rights reserved.