Browsing by Author "Isoglu, Ismail Alper"

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Tuning Mechanical Performance of PCL Scaffolds: Influence of 3D Bioprinting Parameters, Polymer Concentration, and Solvent Selection
(IOP Publishing Ltd, 2025) Ceylan, Saniye Aylin; Baltacioglu, Mehmet Furkan; Bal, Burak; Bayram, Ferdi Caner; Isoglu, Ismail Alper
The mechanical performance of three-dimensional (3D) bioprinted scaffolds is susceptible to printing parameters and material formulation. In this study, poly (epsilon-caprolactone) (PCL) scaffolds were fabricated using four different polymer concentrations (10%, 25%, 50%, and 75% w/v) to investigate how these variations, along with process parameters, influence mechanical behavior. Maintaining the structural integrity of bioprinted constructs requires careful optimization of polymer concentration and precise control over parameters such as printing speed, pressure, and infill density. Tensile tests were conducted to evaluate the effects of these variables. Among the tested conditions, a 50% (w/v) concentration allowed for a broader operational window, enabling fabrication across a range of printing speeds and pressures. At a printing speed of 5 mm s-1, PCL-DCM exhibited a Young's modulus of 39.0 MPa, while PCL-CF samples printed at 10 mm s-1 achieved the highest modulus of 32.0 MPa. Notably, when the printing speed was kept constant, applying higher pressures led to an increase in Young's modulus, suggesting that pressure plays a key role in enhancing scaffold stiffness. When comparing the 50% and 75% (w/v) polymer concentrations, the 50% (w/v) formulation stood out by offering both higher elongation and greater stiffness, which makes it particularly suitable for load-bearing applications. These findings provide a quantitative framework for optimizing extrusion-based bioprinting of PCL scaffolds, with implications for customized biomedical implants and regenerative medicine.
Citation - WoS: 6
Citation - Scopus: 6
3-Sulfopropyl Methacrylate Based Cryogels as Potential Tissue Engineering Scaffolds
(Taylor & Francis Ltd, 2020) Durukan, Adile Yuruk; Isoglu, Ismail Alper
In this study, we developed cryogels containing 3-sulfopropyl methacrylate (SPMA) and 4-vinyl pyridine (4-VP) as a potential scaffold for tissue engineering applications. Cryogels with varying monomer ratios were synthesised by chemical cross-linking under cryogelation conditions. Effect of initiators and cross-linker amount (0.025-0.15 g MBA; 0.012-0.05 g APS; 2.5-12.5 mu l TEMED) and also freezing temperature (-20 and -80oC) were investigated, and the conditions were optimised according to the morphological structures examined by SEM. The functional groups of the materials were characterised by FT-IR. Compression test and swelling were applied to investigate mechanical properties and water absorption ability, respectively. As a preliminary study, selected materials were tested for cell cytotoxicity with MTT. According to our results, the ionic and biocompatible cryogels prepared in this study possessing a highly porous and interconnective structure with good mechanical characteristics and swelling properties can be suitable as tissue scaffolds for many applications.
Citation - WoS: 1
Citation - Scopus: 1
Cinnamomum Zeylanicum Extract Incorporated Electrospun Poly(Lactic Acid)/ Gelatin Membrane as a New Wound Dressing
(Elsevier, 2025) Tarhan, Seray Zora; Pepe, Nihan Aktas; Sen, Alaattin; Isoglu, Ismail Alper
In this study, we fabricated poly(lactic acid)/gelatin electrospun membranes containing various concentrations of Cinnamomum zeylanicum extract and evaluated them as a novel wound dressing. The electrospun membranes were chemically, morphologically, and mechanically characterized, and the results were discussed in comparison with the literature. Electrospun membranes' biodegradability, swelling, and release properties were evaluated, with the CE7.5 membrane having values of 29.60 f 7.20 and 542.1 f 48.3 % and 66.9 %, respectively. Antibacterial activity was observed in CE7.5 and CE10 membranes against E. coli and S. aureus strains. At the highest concentration (CE10), 111.7 f 5.6 % and 96 f 12.375 % cell viability were detected in fibroblasts and differentiated LPS-induced THP-1 cells. Cell viability was further evaluated by Annexin-V/PI staining, revealing that 97.95 f 1.63 % of the cells remained viable in the CE7.5-treated membranes, while only 1.85 f 1.49 % of necrotic cells were detected in the treated cell population. Fibroblasts treated with the CE7.5 membrane showed a 42 % improvement in wound closure compared to non-treated cells. The anti-inflammatory properties of the electrospun membranes were also investigated. Treatment with the conditioned CE7.5 membrane downregulated Tba1 and tau proteins by 45.1 and 51.055 %, respectively. This study concluded that the newly developed Cinnamomum zeylanicum extract incorporated poly(lactic acid)/gelatin electrospun membranes could be a promising wound dressing material.
Citation - WoS: 35
Citation - Scopus: 39
Advances in Micelle-Based Drug Delivery: Cross-Linked Systems
(Bentham Science Publ Ltd, 2017) Isoglu, Ismail Alper; Ozsoy, Yildiz; Isoglu, Sevil Dincer
There are several barriers that drug molecules encounter in body beginning from kidney filtration and reticulo-endothelial system (RES) clearance to cellular trafficking. Multifunctional nanocarriers have a great potential for the delivery of drugs by enhancing therapeutic activity of existing methodologies. A variety of nanocarriers are constructed by different material types, which have unique physicochemical properties for drug delivery applications. Micelles formed by amphiphilic polymers are one of the most important drug/nanocarrier formulation products, in which the core part is suitable for encapsulation of hydrophobic agent whereas the outer shell can be utilized for targeting the drug to the disease area. Micelles as self-assembled nanostructures may encounter difficulties in biodistribution of encapsulated drugs because they have a tendency to be dissociated in dilution or high ionic strength. Therefore, therapeutic efficiency is decreased and it requires high amount of drug to be administered to achieve more efficient result. To overcome this problem, covalently stabilized structures produced by cross-linking in core or shell part, which can prevent the micelle dissociation and regulate drug release, have been proposed. These systems can be designed as responsive systems in which cross-links are degradable or hydrolysable under specific conditions such as low pH or reductive environment. These are enhancing characteristics in drug delivery because their cleavage allows the release of bioactive agent encapsulated in the carrier at a certain site or time. This review describes the chemical methodologies for the preparation of cross-linked micelles, and reports an update of latest studies in literature.
Citation - WoS: 14
Citation - Scopus: 14
Preparation of Antibacterial Electrospun Poly(D, L-Lactide-co-Glycolide)/Gelatin Blend Membranes Containing Hypericum Capitatum Var. Capitatum
(Taylor & Francis As, 2021) Aksit, Nazende Nur; Gurdap, Seda; Isoglu, Sevil Dincer; Isoglu, Ismail Alper
In this study, we fabricated poly(D, L-lactide-co-glycolide)/gelatin (PLGA/gelatin) membranes containing different amounts of Hypericum capitatum var. capitatum (HCC) extract (1, 5, 7.5, 10 wt%) by electrospinning technique. We investigated chemical, morphological, physical, and mechanical properties as well as in vitro degradation behavior of the electrospun membranes. We also evaluated the antibacterial activity of the electrospun membranes against Escherichia coli and Staphylococcus aureus. Viability, adhesion, and attachment of human fibroblast cells on the electrospun membranes on pre-set days were evaluated by the colorimetric CellTiter 96(R) AQueous One Solution Cell Proliferation Assay (MTS assay), scanning electron microscopy (SEM), and 4',6-Diamidino-2-Phenylindole (DAPI) staining.
Citation - WoS: 12
Citation - Scopus: 12
Sulfobetaine-Based Homo- and Copolymers by Raft: Cross-Linked Micelles and Aqueous Solution Properties
(Amer Chemical Soc, 2022) Gurdap, Seda; Bayram, Nazende Nur; Isoglu, Ismail Alper; Isoglu, Sevil Dincer
In this study, we describe the synthesis and aqueous solution behavior of temperature-sensitive N-(3-sulfopropyl)-N-methacroyloxyethyl-N,N-dimethylammonium betaine (SBMA) homopolymers and core cross-linked micelles (CCMs) with an SBMA shell. Reversible addition- fragmentation chain transfer polymerization has been utilized to synthesize sulfobetaine homopolymers, followed by CCM formation during copoly-merization in the presence of an acid-degradable cross-linker. First, SBMA homopolymers of varying chain lengths were synthesized, and it has been demonstrated that an increase in the chain length and concentration of the homopolymer resulted in an increase in the upper critical solution temperature (UCST). Besides, micelles showed concentration-dependent dual temperature-sensitive behavior with UCST and LCST transitions. Also, homopolymers and CCMs were characterized by FTIR, H-1-NMR, GPC, and TEM. Micelle formation and temperature sensitivity were also investigated by DLS. As a result, stabilized micelles were successfully prepared with the motivation of preventing premature drug release and achieving a pH-and temperature-controlled system. Due to their dual-responsive characteristics, the CCMs show promising potential to be used as smart drug carriers for controlled delivery.
Citation - WoS: 10
Citation - Scopus: 12
On the Detailed Mechanical Response Investigation of PHBV/PCL and PHBV/PLGA Electrospun Mats
(IOP Publishing Ltd, 2019) Bal, Burak; Tugluca, Ibrahim Burkay; Koc, Nuray; Isoglu, Ismail Alper
In this study, electrospun mats of pristine poly(epsilon-caprolactone) (PCL), Poly(D, L-lactide-co-glycolide) (PLGA), poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), as well as PHBV/PCL blends and PHBV/PLGA blends in different ratios (80:20, 75:25, 50:50, 25:75, 20:80, 10:90, 5:95%, w/w) and Centella Asiatica (CA) loaded (1, 5, 10%, w/v) PHBV/PCL and PHBV/PLGA polyester blends were prepared. Electrospun mats were characterized by scanning electron microscopy (SEM) in order to show uniform and bead and defect-free fiber structure with average diameter. The blend ratio and strain rate dependencies of mechanical behavior of these electrospun membranes were investigated under tensile loading. The tensile tests were conducted at an initial strain rates of 10(-1) s(-1), 10(-2) s(-1), 10(-3) s(-1) and 10(-4) s(-1) at room temperature and the best and worst combinations of PHBV/PLGA, PHBV/PCL blend ratios for both stress and ductility required applications were specified for each strain rate. The effects of blend ratios on the tensile strength and Young's modulus were also investigated. Moreover, the effects of Centella Asiatica on the electrospun membranes' mechanical behavior were demonstrated at different strain rates. Consequently, this study constitutes an important guideline for the selection and usage of the aforementioned electrospun membranes as a wound dressing material in terms of mechanical response at different loading scenarios.
Citation - WoS: 3
Citation - Scopus: 3
HER2-Specific Peptide (LTWWYSPY) and Antibody (Herceptin) Targeted Core Cross-Linked Micelles for Breast Cancer: A Comparative Study
(MDPI, 2023) Bayram, Nazende Nur; Ulu, Gizem Tugce; Abdulhadi, Nusaibah Abdulsalam; Guerdap, Seda; Isoglu, Ismail Alper; Baran, Yusuf; Isoglu, Sevil Dincer
This study aims to prepare a novel breast cancer-targeted micelle-based nanocarrier, which is stable in circulation, allowing intracellular drug release, and to investigate its cytotoxicity, apoptosis, and cytostatic effects, in vitro. The shell part of the micelle is composed of zwitterionic sulfobetaine ((N-3-sulfopropyl-N,N-dimethylamonium)ethyl methacrylate), while the core part is formed by another block, consisting of AEMA (2-aminoethyl methacrylamide), DEGMA (di(ethylene glycol) methyl ether methacrylate), and a vinyl-functionalized, acid-sensitive cross-linker. Following this, a targeting agent (peptide (LTVSPWY) and antibody (Herceptin((R)))), in varying amounts, were coupled to the micelles, and they were characterized by H-1 NMR, FTIR (Fourier-transform infrared spectroscopy), Zetasizer, BCA protein assay, and fluorescence spectrophotometer. The cytotoxic, cytostatic, apoptotic, and genotoxic effects of doxorubicin-loaded micelles were investigated on SKBR-3 (human epidermal growth factor receptor 2 (HER2)-positive) and MCF10-A (HER2-negative). According to the results, peptide-carrying micelles showed a higher targeting efficiency and better cytostatic, apoptotic, and genotoxic activities than antibody-carrying and non-targeted micelles. Also, micelles masked the toxicity of naked DOX on healthy cells. In conclusion, this nanocarrier system has great potential to be used in different drug-targeting strategies, by changing targeting agents and drugs.
Engineering a Bilayered Scaffold as a Potential Cardiac Patch: From Scaffold Design to in Vitro Assessment
(Springer Singapore Pte Ltd, 2025) Yuruk, Adile; Duzler, Ayhan; Isoglu, Sevil Dincer; Isoglu, Ismail Alper
In this study, we developed a novel bilayered scaffold consisting of a bottom layer composed of the Decellularized Bovine Pericardium (DP) coated with Polyaniline Nanoparticles (PANINPs) and a top layer made of an electrospun Poly(lactic-co-glycolic acid)/Gelatin (PLGA/Gel) membrane incorporated with Vascular Endothelial Growth Factor (VEGF) and hawthorn extract. Functionally, the DP supplies native Extracellular Matrix (ECM) components and mechanical support, while PANINPs provide conductivity. The electrospun PLGA/Gel layer mimics fibrous ECM. It incorporates bioactives, with VEGF promoting pro-angiogenic stimulation and hawthorn extract enhancing anticoagulant activity, as well as increasing surface hydrophilicity. The tissue adhesive ensures the interfacial integrity between the two layers. Decellularization efficiency was confirmed histologically using 4 ',6-diamidino-2-phenylindole (DAPI) and Hematoxylin-Eosin (H&E) staining. The DP exhibited a DNA content of 115.9 +/- 47.8 ng/mg DNA, compared to 982.88 +/- 395.42 ng/mg in Native Pericardium (NP). The PANINPs had an average particle size of 104.94 +/- 13.7 nm. The conductivity of PANINPs-coated decellularized pericardium was measured to be 9.093 +/- 8.6 x 10- 4 S/cm using the four-point probe method. PLGA/Gel membranes containing hawthorn extract (1%, 5%, 10%, and 15% w/v) and VEGF (0.1 mu g/mL, 0.5 mu g/mL, and 1 mu g/mL) were fabricated by electrospinning, resulting in fiber diameters between 850 and 1200 nm and pore sizes between 14 and 20 mu m. The anticoagulant efficiency of the membranes containing hawthorn extract reached 430 s in the Activated Partial Thromboplastin Time Assay (aPTT). Mechanical testing revealed a tensile strength of 22.70 +/- 6.33 MPa, an elongation of 53.58 +/- 10.63%, and Young's modulus of 0.67 +/- 0.10 MPa. The scaffold also exhibited over 91% cell viability and excellent cardiomyocyte adhesion. The hemolysis ratio was determined to be 0.421 +/- 0.191%, which confirms its blood compatibility. Our results indicate that the proposed bilayered scaffold can be a promising candidate for cardiac patch applications.
A Potential Hemostatic Chitosan/Gelatin Cryogel Impregnated with Verbascum Thapsus Leaf Extract for Noncompressible Hemorrhage Management
(IOP Publishing Ltd, 2025) Uzuner, Hacernur; Yuruk, Adile; Isoglu, Ismail Alper
In this study, we prepared a series of chitosan/gelatin (CS/GEL) cryogels containing Verbascum thapsus (V. thapsus) leaf extract and identified a lead formulation for noncompressible hemorrhage (NCH). Cryogels with average pore diameters ranging from 225 to 478 mu m were fabricated through cryogelation at various CS/GEL ratios. C15 was chosen as the base scaffold due to its homogeneous pore distribution, with a pore size coefficient of variation (CV) of approximately 0.22. Extract loading was 1%, 5%, 10%, and 20% w/v. Functional porosity was reported by the relative accessible void index (RAVI). In PBS, the values relative to neat C15 were 1.00, 0.27, 0.20, 0.13, and 0.09 for concentrations of 0%, 1%, 5%, 10%, and 20% w/v, respectively. In citrated blood, the series was 1.00, 0.29, 0.12, 0.14, and 0.09. After loading, equilibrium swelling decreased and the compressive modulus increased, consistent with partial pore filling in a fixed network. The cryogels maintained an interconnected macroporous network and showed swelling from 300% to 3600% in blood and PBS. Antibacterial activity reached 89% inhibition, and cell viability remained above 80%. Hemolysis was low and within acceptance limits. Clotting improved in whole blood as the blood clotting index decreased from 11.9 to 6.5, and the clotting time was approximately 6 min. The 5% w/v group provided the optimal balance of clotting, antibacterial effects, and biocompatibility. This study presents a novel hemostatic CS/GEL cryogel containing V. thapsus leaf extract that holds strong potential for future applications in NCH management.
Citation - WoS: 8
Citation - Scopus: 8
RAFT-Mediated Synthesis of Poly( N-(2-Hydroxypropyl)Methacrylamide-b-4-vinylpyridine)by Conventional and Microwave Heating
(Springer, 2013) Ozdemir, Zeynep; Topuzogullari, Murat; Isoglu, Ismail Alper; Dincer, Sevil
We report the synthesis of N-(2-hydroxypropyl)methacrylamide (HPMA) macroCTA and HPMA-b-4-Vinylpyridine block copolymers via reversible addition-fragmentation chain transfer (RAFT) reaction. Polymerization was carried out in dimethylformamide (DMF) at 70 A degrees C using 4-Cyano-4(thiobenzoylthio) pentanoic acid as chain transfer agent and AIBN as an initiator. Control over molecular weight and composition was achieved by altering the CTA, monomer and initiator feed ratio. The controlled living character of the polymerization was verified with pseudo-first-order kinetic plots, a linear increase of the molecular weight with conversion, and low polydispersities (PDIs a parts per thousand currency sign 1.2). Effect of microwave heating on the homo- and copolymer formation was investigated and the rates were significantly higher than those observed under conventional heating conditions. These polymerization reactions were in controlled fashion resulting in polymers with low PDIs, too. These polymers have a great potential to be used in developing delivery vehicles and conjugates for further drug or gene delivery applications.
Citation - WoS: 5
Citation - Scopus: 5
Centella Asiaticaextract Containing Bilayered Electrospun Wound Dressing
(Korean Fiber Soc, 2020) Isoglu, Ismail Alper; Koc, Nuray
Innovative and bioactive wound dressings prepared by electrospinning mimicking the native structure of the extracellular matrix (ECM) have gained significant interest as an alternative to conventional wound care applications. In this study, bilayered wound dressing material was produced by sequential electrospinning of quaternized poly(4-vinyl pyridine) (upper layer) on theCentella Asiatica(CA) extract containing electrospun poly(D, L-lactide-co-glycolide) (PLGA)/poly(3-hydroxybutyrate-co-3-hydroxy valerate) (PHBV) blend membrane (lower layer). Scanning electron microscopy (SEM) was utilized to show a uniform and bead-free fiber structure of electrospun membranes. The average diameter of CA extract containing electrospun PLGA/PHBV blend membrane was calculated 0.471 +/- 0.11 mu m, whereas the average fiber diameter of electrospun poly(Q-VP) membranes was in the range of 0.460 +/- 0.057 mu m. Chemical, thermal, mechanical properties, and adsorption capacity of electrospun membranes, as well as the cumulative release of CA from the electrospun PLGA/PHBV membrane, were investigated. Viability, adhesion, and attachment of human fibroblast cells on the electrospun membranes on pre-set days were evaluated by the colorimetric CellTiter 96 (R) Aqueous One Solution Cell Proliferation Assay (MTS assay) and SEM. Results revealed that CA loaded bilayered electrospun wound dressing showed promoted attachment and proliferation of fibroblasts. Hence, it can be concluded that CA extract containing bilayered electrospun wound dressing prepared in this study has a promising potential for wound healing applications.
Peptide Targeted Core Cross-Linked Micelles for Dox Delivery to HER2 Expressing Cancer Cells
(Mary Ann Liebert, inc, 2022) Bayram, Nazende Nur; Ulu, Gizem Tugce; Gurdap, Seda; Isoglu, Ismail Alper; Baran, Yusuf; Isoglu, Sevil Dincer
Citation - WoS: 8
Citation - Scopus: 11
Stem Cells Combined 3D Electrospun Nanofibrous and Macrochannelled Matrices: A Preliminary Approach in Repair of Rat Cranial Bones
(Taylor & Francis Ltd, 2019) Isoglu, Ismail Alper; Bolgen, Nimet; Korkusuz, Petek; Vargel, Ibrahim; Celik, Hakan Hamdi; Kilic, Emine; Piskin, Erhan
Repair of cranial bone defects is an important problem in the clinical area. The use of scaffolds combined with stem cells has become a focus in the reconstruction of critical-sized bone defects. Electrospinning became a very attracting method in the preparation of tissue engineering scaffolds in the last decade, due to the unique nanofibrous structure of the electrospun matrices. However, they have a limitation for three dimensional (3D) applications, due to their two-dimensional structure and pore size which is smaller than a cellular diameter which cannot allow cell migration within the structure. In this study, electrospun poly(epsilon-caprolactone) (PCL) membranes were spirally wounded to prepare 3D matrices composed of nanofibers and macrochannels. Mesenchymal stromal/stem cells were injected inside the scaffolds after the constructs were implanted in the cranial bone defects in rats. New bone formation, vascularisation and intramembranous ossification of the critical size calvarial defect were accelerated by using mesenchymal stem cells combined 3D spiral-wounded electrospun matrices.
Citation - WoS: 6
Citation - Scopus: 5
Raft-Synthesized Poegma-B Block Copolymers: Preparation of Nanosized Micelles for Anticancer Drug Release
(Springer, 2022) Bayram, Nazende Nur; Topuzogullari, Murat; Isoglu, Ismail Alper; Isoglu, Sevil Dincer
To achieve high stability and biocompatibility in physiological environment, oligoethyleneglycol methacrylate (OEGMA) and 4-vinylpyridine (4VP)-based amphiphilic block copolymers were prepared as micellar carriers to deliver doxorubicin into tumor cells. First, macroinitiator of OEGMA was synthesized by RAFT polymerization at [M](0)/[CTA](0)/[I](0) ratio of 100/1/0.2 in dimethylformamide (DMF) at 70 degrees C, in the presence of 4,4'-azobis(4-cyanovaleric acid) (ACVA) as initiator and 4-cyano-4-(thiobenzoylthio)pentanoic acid (CTA) as chain transfer agent, respectively. It was followed by copolymerization with 4-VP at similar conditions. The formation of RAFT-mediated polymers was approved by FTIR, H-1-NMR and GPC. For the preparation of drug-loaded micelles, a dialysis method was applied and hydrophobic doxorubicin, as a model drug, was entrapped into the micelles. Size distributions and morphologies of drug-loaded micelles were investigated by light scattering and scanning electron microscopy, respectively. Critical micelle concentration was estimated as 0.0019 mg/mL by measuring light scattering intensity in different polymer concentrations. Also, drug loading and entrapment efficiencies were calculated as 4.41% and 17.65% by measuring the DOX amount in the micelles, spectrophotometrically. At last, the drug-loaded micelles were applied to SKBR-3 breast cancer cell lines and revealed up to %40 cell inhibition at 48 and 72 h. As a result, these nanosized and biocompatible micelles can be used for the delivery of hydrophobic drugs, and they can also be modified for further targeting and imaging applications toward specific cancer cells. [GRAPHICS] .
Citation - WoS: 3
Citation - Scopus: 3
Pericardial Fluid and Vascular Tissue Engineering: A Preliminary Study
(Ios Press, 2021) Sonmezer, Dilek; Latifoglu, Fatma; Toprak, Guler; Duzler, Ayhan; Isoglu, Ismail Alper
BACKGROUND: The heart is surrounded by a membrane called pericardium or pericardial cavity. OBJECTIVE: In this study, we investigated the pericardial fluid (PF) for coating polycaprolactone (PCL) scaffolds. PFS, which is a PF component, was used for the coating material. In addition to using PFS for surface coating, MED and fetal bovine serum (FBS) were also used for comparison. METHODS: Pericardial fluid cells (PFSc) isolated from PF were cultured on coated PCL scaffolds for 1, 3, and 5 days. Cell viability was determined using 3-(4, 5-di-methylthiazol- 2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. RESULTS: The MTT assay results showed that the viability of cells on PCL scaffold coated with PFS increased over time (P < 0.005), and cell viability was significantly different between PCL scaffolds coated with PFS and non-coated PCL scaffolds. However, cell viability was significantly higher in the PCL scaffolds coated with PFS than non-coated and coated with FBS, MED, and PCL scaffolds. Scanning electron microscopy (SEM) microscopy images and MTT assay indicated that PFSc are attached, proliferated, and spread on PCL scaffolds, especially on PCL scaffolds coated with PFS. CONCLUSIONS: These results suggest that PFS is a biocompatible material for surface modification of PCL scaffolds, which can be used as a suitable material for tissue engineering applications.
Citation - WoS: 4
Citation - Scopus: 3
Preparation and Characterization of Viburnum Opulus Containing Electrospun Membranes as Antibacterial Wound Dressing
(Korean Fiber Soc, 2023) Yuruk, Adile; Isoglu, Sevil Dincer; Isoglu, Ismail Alper
Herein, we fabricated polycaprolactone/gelatin electrospun membranes possessing different amounts of Viburnum Opulus extract (0, 25, 35, 50%, w/v) as an antibacterial wound dressing. We investigated chemical, morphological, physical, and mechanical properties as well as in vitro degradation behavior of the electrospun membranes. The antibacterial activities of membranes were evaluated against gram-positive Staphylococcus aureus (S. aureus) and gram-negative Escherichia coli (E. coli). The membranes containing Viburnum Opulus exhibited excellent antibacterial activity with the formation of inhibition zones of 25 mm to 36 mm against Escherichia coli and 14 mm to 25 mm against Staphylococcus aureus. The fiber diameters rose from 591 to 1222 nm after adding Viburnum Opulus extract. The extract-containing membranes displayed superior swelling, cell viability, and proliferation properties to neat membranes. Our results showed that the polycaprolactone/gelatin electrospun membranes containing Viburnum Opulus could be a suitable material for wound dressing applications.