Acar, Mustafa BurakAyaz-Guner, SerifeDi Bernardo, Giovanni D.Guner, HüseyinMurat, AyşegülPeluso, G. F.Galderisi, Umberto2025-09-252025-09-2520201945-4589https://doi.org/10.18632/aging.202423https://hdl.handle.net/20.500.12573/4303Acar, Mustafa Burak/0000-0002-9109-6575; Ayaz Guner, Serife/0000-0002-1052-0961; Murat-Cifci, Aysegul/0000-0002-6823-4175; Guner, Huseyin/0000-0002-0220-5224; Ozcan, Servet/0000-0002-9914-8843; Di Bernardo, Giovanni/0000-0002-4985-4029;The mesenchymal stromal cells (MSCs) residing within the stromal component of visceral adipose tissue appear to be greatly affected by obesity, with impairment of their functions and presence of senescence. To gain further insight into these phenomena, we analyzed the changes in total proteome content and secretome of mouse MSCs after a high-fat diet (HFD) treatment compared to a normal diet (ND). In healthy conditions, MSCs are endowed with functions mainly devoted to vesicle trafficking. These cells have an immunoregulatory role, affecting leukocyte activation and migration, acute inflammation phase response, chemokine signaling, and platelet activities. They also present a robust response to stress. We identified four signaling pathways (TGF-β, VEGFR2, HMGB1, and Leptin) that appear to govern the cells’ functions. In the obese mice, MSCs showed a change in their functions. The immunoregulation shifted toward pro-inflammatory tasks with the activation of interleukin-1 pathway and of Granzyme A signaling. Moreover, the methionine degradation pathway and the processing of capped intronless pre-mRNAs may be related to the inflammation process. The signaling pathways we identified in ND MSCs were replaced by MET, WNT, and FGFR2 signal transduction, which may play a role in promoting inflammation, cancer, and aging © 2024 Elsevier B.V., All rights reserved.eninfo:eu-repo/semantics/openAccessMesenchymal Stromal CellsSenescenceVisceral Adipose TissueMethionineGranzymesHmgb1 ProteinInterleukin-1LeptinMethionineProteomeProto-Oncogene Proteins C-MetReceptor, Fibroblast Growth Factor, Type 2Rna PrecursorsTransforming Growth Factor BetaVascular Endothelial Growth Factor Receptor-2Fibroblast Growth Factor Receptor 2GranzymeHigh Mobility Group B1 ProteinInterleukin 1LeptinMethionineProteomeRna PrecursorScatter Factor ReceptorTransforming Growth Factor BetaVasculotropin Receptor 2AgingAnimalCytologyInflammationIntra-Abdominal FatLipid DietMesenchymal Stem CellMetabolismMouseObesityRna ProcessingSecretory VesicleSignal TransductionWnt SignalingAgingAnimalsDiet, High-FatGranzymesHmgb1 ProteinInflammationInterleukin-1Intra-Abdominal FatLeptinMesenchymal Stem CellsMethionineMiceObesityProteomeProto-Oncogene Proteins C-MetReceptor, Fibroblast Growth Factor, Type 2Rna PrecursorsRna Processing, Post-TranscriptionalSecretory VesiclesSignal TransductionTransforming Growth Factor BetaVascular Endothelial Growth Factor Receptor-2Wnt Signaling PathwayArticle10.18632/aging.2024232-s2.0-85099059270