Turan, Merve GulKantarci, HanifeCevik, SebihaKaplan, Oktay I.2025-09-252025-09-2520252589-0042https://doi.org/10.1016/j.isci.2025.111791https://hdl.handle.net/20.500.12573/3179Kaplan, Oktay Ismail/0000-0002-8733-0920;The interaction of cilia with various cellular compartments, such as axons, has emerged as a new form of cellular communication. Cilia often extend in proximity to cilia from neighboring cells. However, the mechanisms driving this process termed juxtaposed cilia-cilia elongation (JCE) remain unclear. We use fluorescence-based visualization to study the mechanisms of coordinated cilia elongation in sensory neurons of Caenorhabditis elegans. Conducting a selective gene-based screening strategy reveals that ARL-13/ARL13B and MKS-5/RPGRIP1L are essential for JCE. We demonstrate that ARL-13 modulates JCE independently of cilia length. Loss of NPHP-2/inversin along with HDAC-6 enhances the cilia misdirection phenotype of arl-13 mutants, while disruption of the BBSome complex, but not microtubule components, partially suppresses the JCE defects in arl-13 mutants. We further show changes in the phospholipid compositions in arl-13 mutants. We suggest that ARL-13 contributes to JCE, in part, through the modulation of the ciliary membrane.eninfo:eu-repo/semantics/openAccessArticle10.1016/j.isci.2025.1117912-s2.0-85215584381