Saraymen, EsmaErdem, YakutAkalin, Hilal ÜnlüTaşçıoğlu, NazifeSaraymen, BerkayCelik, SerhatÖzkul, Yusuf T.2025-09-252025-09-2520240145-56801165-158Xhttps://doi.org/10.14715/cmb/2024.70.11.5https://hdl.handle.net/20.500.12573/3801Homeobox (HOX) transcript antisense RNA (HOTAIR) and HOX genes are reported to be more expressed in various cancers in humans in recent studies. The role of HOTAIR and HOXD genes in acute myeloid leukemia (AML) and chronic myeloid leukemia (CML) is not well known. In this study, expression levels of HOXD8, HOXD9 and HOXD11 from HOXD gene family and HOTAIR were determined from peripheral blood samples of 30 AML and 30 CML patients and 20 healthy volunteers by quantitative Real Time PCR. We determined that the expression levels of HOXD9 and HOXD11 in the AML patients were significantly lower than the control group (p<0.001 and p=0.002, respectively). There was no significant difference in the expression levels of HOTAIR and HOXD8 when compared to the control group. In the CML patients there was a significant increase in the expression level of HOTAIR when compared to the control group (p=0.002). The expression levels of HOXD9 and HOXD11 were found to be significantly lower than the control group (p<0.001). Our study showed that HOTAIR may not be a biomarker in the diagnosis and is not significantly correlated with the clinicopathological prognostic characteristics of AML. Additionally; it can be said that HOTAIR is oncogenic by suppressing the expression of HOXD9 and HOXD11 but not HOXD8 in CML patients. The expression profiles of HOTAIR may be a potential biomarker in the diagnosis of CML patients in predicting and monitoring drug resistance. © 2025 Elsevier B.V., All rights reserved.eninfo:eu-repo/semantics/closedAccessAmlCmlHotairHoxd11Hoxd8Hoxd9ImatinibNilotinibHomeodomain ProteinsHotair Long Untranslated Rna, HumanHoxd8 Protein, HumanHoxd9 Protein, HumanNeoplasm ProteinsRna, Long NoncodingTranscription FactorsImatinibNilotinibHomeodomain ProteinHotair Long Untranslated Rna, HumanHoxd8 Protein, HumanHoxd9 Protein, HumanLong Untranslated RnaTranscription FactorTumor ProteinAcute Myeloid LeukemiaAdultArticleBlood SamplingCancer PatientCancer PrognosisChronic Myeloid LeukemiaClinical FeatureControlled StudyFemaleGene Expression LevelHistopathologyHotair GeneHox GeneHoxd11 GeneHoxd8 GeneHoxd9 GeneHumanMajor Clinical StudyMaleMiddle AgedNonhumanReal Time Polymerase Chain ReactionTurkey (Republic)AdolescentAgedBloodCase Control StudyGene Expression RegulationGeneticsMetabolismTurkey (Bird)Young AdultAdolescentAdultAgedCase-Control StudiesFemaleGene Expression Regulation, LeukemicHomeodomain ProteinsHumansLeukemia, Myelogenous, Chronic, Bcr-Abl PositiveLeukemia, Myeloid, AcuteMaleMiddle AgedNeoplasm ProteinsRna, Long NoncodingTranscription FactorsTurkeyYoung AdultArticle10.14715/cmb/2024.70.11.52-s2.0-85213024791