Sonmezer, DilekLatifoglu, FatmaToprak, GulerDuzler, AyhanIsoglu, Ismail Alper2021-12-152021-12-1520210959-29891878-3619PubMed ID33682690https //doi.org/10.3233/BME-196014https://hdl.handle.net/20.500.12573/1077This work was supported by the Scientific Research Projects Unit of Erciyes University with the project ONAP, FOA-2016-6692, Turkey.BACKGROUND: The heart is surrounded by a membrane called pericardium or pericardial cavity. OBJECTIVE: In this study, we investigated the pericardial fluid (PF) for coating polycaprolactone (PCL) scaffolds. PFS, which is a PF component, was used for the coating material. In addition to using PFS for surface coating, MED and fetal bovine serum (FBS) were also used for comparison. METHODS: Pericardial fluid cells (PFSc) isolated from PF were cultured on coated PCL scaffolds for 1, 3, and 5 days. Cell viability was determined using 3-(4, 5-di-methylthiazol- 2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. RESULTS: The MTT assay results showed that the viability of cells on PCL scaffold coated with PFS increased over time (P < 0.005), and cell viability was significantly different between PCL scaffolds coated with PFS and non-coated PCL scaffolds. However, cell viability was significantly higher in the PCL scaffolds coated with PFS than non-coated and coated with FBS, MED, and PCL scaffolds. Scanning electron microscopy (SEM) microscopy images and MTT assay indicated that PFSc are attached, proliferated, and spread on PCL scaffolds, especially on PCL scaffolds coated with PFS. CONCLUSIONS: These results suggest that PFS is a biocompatible material for surface modification of PCL scaffolds, which can be used as a suitable material for tissue engineering applications.enginfo:eu-repo/semantics/closedAccesstissue engineeringpericardial fluid structurepolycaprolactone (PCL)Pericardial fluid (PF)articleVolume 32 Issue 2 Page 101-113