Sulak, MineTurgut, Gurbet ÇelikSen, Alaattin2025-09-252025-09-2520221612-18801612-1872https://doi.org/10.1002/cbdv.202200131https://hdl.handle.net/20.500.12573/3431In this study, cerium oxide nanoparticles (CONPs) were synthesized using fresh green walnut shell extract in microwave environment. The morphology and structure of the CONPs were determined using ultraviolet-visible (UV/VIS), attenuated total reflection-Fourier transform infrared (ATR-FT-IR), X-ray diffraction (XRD), energy-dispersive X-ray (EDX) spectroscopy, and scanning electron microscopy (SEM). Crystal purple staining, Annexin V-FITC detection, RT-PCR, P53, and NF-κB luciferase reporter assays were performed to evaluate the mechanism of action of CONPs in breast cancer cell lines (MCF7). The biosynthesized CONPs showed cytotoxic effects and induced apoptosis in MCF7 cells. Furthermore, CONPs induced P53 expression and suppressed NF-κB gene expression, both of which were confirmed using reporter assays. Based on the present results, it was concluded that CONPs can induce apoptosis by acting on P53 at the transcriptional level and may cause cell death by suppressing NF-κB-mediated transcription. © 2022 Elsevier B.V., All rights reserved.eninfo:eu-repo/semantics/closedAccessApoptosisBreast CancerCerium Oxide NanoparticlesNf-ΚbP53CeriumCeric OxideCeriumNf-Kappa BPlant ExtractsTumor Suppressor Protein P53Cerium Oxide NanoparticleImmunoglobulin Enhancer Binding ProteinProtein P53Ceric OxideCeriumMetal NanoparticlePlant ExtractAntineoplastic ActivityApoptosisArticleAttenuated Total Reflectance Fourier Transform Infrared SpectroscopyControlled StudyCytotoxicityDrug MechanismDrug StructureDrug SynthesisEnergy Dispersive X Ray SpectroscopyGene ExpressionHumanHuman CellIC50Luciferase AssayMCF-7 Cell LineMicrowave CookingNutshell (Structure)Particle SizeReverse Transcription Polymerase Chain ReactionScanning Electron MicroscopyUltraviolet Visible SpectroscopyWalnutX Ray DiffractionBreast TumorChemistryFemaleGeneticsInfrared SpectroscopyMicrowave RadiationBreast NeoplasmsCeriumFemaleHumansJuglansMetal NanoparticlesMicrowavesNf-Kappa BPlant ExtractsSpectroscopy, Fourier Transform InfraredTumor Suppressor Protein P53X-Ray DiffractionArticle10.1002/cbdv.2022001312-s2.0-85133952780