Şansaçar, MerveGencer Akçok, Emel Başak2025-09-252025-09-25202597815974529469781617792304978161779766815974557419781603272476978159745303597814939122309781588298645978161779339497816177996481064-37451940-6029https://doi.org/10.1007/7651_2024_526https://hdl.handle.net/20.500.12573/4175Autophagy is an evolutionarily conserved process providing the energy that cells need to survive, especially in stress situations, through catabolic processes. Considering the dual role of autophagy in cancer cells depending on the cellular context, it is crucial to comprehend the effect of drug candidates put forward to prevent cancer through the autophagy pathway. The CYTO-ID® Autophagy Detection Kit allows a rapid, specific and quantitative measurement of autophagic activity at the cellular level using a 488 nm-excitable green fluorescent detection reagent via flow cytometer. In this chapter, we present the CYTO-ID® Autophagy Detection method with a stepwise protocol to monitor the autophagy flux after the application of any compound to suspension cancer cell lines with flow cytometric analysis. © 2025 Elsevier B.V., All rights reserved.eninfo:eu-repo/semantics/closedAccessAutophagic CompartmentsAutophagic FluxAutophagosomeAutophagyCancerCyto-IdFlow CytometryChloroquineSirolimusFluorescent DyesCyto-IdChloroquineReagentSirolimusFluorescent DyeAutolysosomeAutophagosomeAutophagy (Cellular)Cancer CellCell CultureFlow CytometryFluorescence Activated Cell SortingFluorescence IntensityHumanHuman CellChemistryMetabolismNeoplasmPathologyProceduresStainingTumor Cell LineAutophagyCell Line, TumorFlow CytometryFluorescent DyesHumansNeoplasmsStaining and LabelingBook Part10.1007/7651_2024_5262-s2.0-85219494510