Iplik, ElifErtugrul, BarisKozanoglu, İlknurBaran, YusufCakmakoglu, Bedia2019-06-272019-06-272018IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES Volume: 21 Issue: 5 Pages: 465-468 DOI: 10.22038/IJBMS.2018.26152.64202008-3866e-2008-3874PubMed ID: 29922425Accession Number: WOS:000433360100004http://acikerisim.agu.edu.tr/xmlui/handle/20.500.12573/42Objective(s): Colon cancer is risen up with its complex mechanism that directly impacts on its treatment as well as its common prevalence. Mesenchymal stem cells (MSCs) have been considered as a therapeutic candidate for conventional disease including cancer. In this research, we have focused on apoptotic effects of adipose tissue-derived MSCs in colon cancer. Materials and Methods: MSCs were obtained from adipose tissue and characterized by Flowcytometer using suitable antibodies. MSCs, HT-29, HCT-116, RKO and healthy cell line MRC5 were cultured by different seeding procedure. After cell viability assay, changes in caspase 3 enzyme activity and the level of phosphatidylserine were measured. Results: For cell viability assay, a 48 hr incubation period was chosen to seed all cells together. There was a 1.36-fold decrease in caspase 3 enzyme activity by co-treatment of RKO and MSCs in addition to 2.02-fold decrease in HT-29 and MSCs co-treatment, and 1.103-fold increase in HCT-116 and MSCs. The results demonstrated that HCT-116 led to the highest rate of apoptotic cell death (7.5%) compared with other cells. Conclusion: We suggest that MSCs might remain a new treatment option for cancer by its differentiation and repair capacity.enginfo:eu-repo/semantics/openAccessApoptosisColon cancerStem cellsCell deatharticle