Icoz, KutayGercek, TayyibeMurat, AysegulOzcan, ServetUnal, Ekrem2019-07-302019-07-302019BIOTECHNOLOGY PROGRESS Volume: 35 Issue: 1 Article Number: UNSP e2737 DOI: 10.1002/btpr.27378756-7938eISSN: 1520-6033Accession Number: WOS:000458312200028DOI: 10.1002/btpr.2737Article Number: UNSP e2737http://acikerisim.agu.edu.tr/xmlui/handle/20.500.12573/76Authors acknowledge The Scientific and Technological Research Council of Turkey (TUBITAK Project No: 115E020) for financial support and Prof. Ahmet Eken and Prof. Musa Karakukcu from Erciyes University for valuable discussions for the cell line and immunomagnetic separation related issues. Ekrem Unal acknowledges Gilead Research Scholarship program.One way to monitor minimal residual disease (MRD) is to screen cells for multiple surface markers using flow cytometry. In order to develop an alternative microfluidic based method, isolation of B type acute lymphoblastic cells using two types of antibodies should be investigated. The immunomagnetic beads coated with various antibodies are used to capture the B type acute lymphoblastic cells. Single beads, two types of beads and surface immobilized antibody were used to measure the capture efficiency. Both micro and nanosize immunomagnetic beads can be used to capture B type acute lymphoblastic cells with a minimum efficiency of 94% and maximum efficiency of 98%. Development of a microfluidic based biochip incorporating immunomagnetic beads and surface immobilized antibodies for monitoring MRD can be an alternative to current cost and time inefficient laboratory methods. (c) 2018 American Institute of Chemical Engineers Biotechnol. Prog., 35: e2737, 2019enginfo:eu-repo/semantics/closedAccessB type acute lymphoblastic leukemia cellscell capture efficiencyminimal residual diseaseimmunomagnetic separationtwo antibody sortingarticle10.1002/btpr.2737