Demirci, Muserref Duygu SacarOrhan, Mehmet EminErginkoc, Altay NidaSaçar Demirci, Müşerref Duygu2026-04-212026-04-2120261476-92711476-928Xhttps://hdl.handle.net/20.500.12573/5918https://doi.org/10.1016/j.compbiolchem.2026.109002West Nile virus (WNV) is a mosquito-borne flavivirus linked to severe neuroinvasive disease. Although host and vector microRNAs (miRNAs) have been implicated in viral infection, the presence and functional relevance of WNV-encoded miRNAs remain largely unexplored. Here, we developed an integrative bioinformatics pipeline that combines multiple miRNA prediction algorithms with secondary structure screening and host transcriptomic data to identify high-confidence candidate WNV-derived mature miRNAs. Overlap-based confidence scoring and differential expression support from RNA-seq datasets prioritized a small subset of putative miRNA-mRNA interactions with potential roles in infection-associated gene regulation. A competitive endogenous RNA network constructed from predicted mRNA, lncRNA, and circRNA targets highlighted pathways involving innate immunity, GPCR and Wnt signaling, RNA degradation, and viral replication. Together, these findings provide a reproducible computational workflow and nominate testable regulatory interactions for future experimental validation.eninfo:eu-repo/semantics/closedAccessMicrornaVirusesDiseaseRNAWest Nile VirusArticle10.1016/j.compbiolchem.2026.1090022-s2.0-105034747512